(using henderson hassel bach equation) Describe in detail how you would make 500mL of a 250mM buffer at pH 9.0 from the following list of chemicals: 1M NaOH. 1M HCl. Ammonium chloride salt (solid). 30% Ammonia (liquid). For your buffer preparation give all species present and their concentrations in solution. first use henderson hasselbach equation to find the relation between weak electrolyte and its conjugate ion.The buffer concentration is 250 mM. This means that the total concentration of the weak electrolyte and its conjugate base is 100 mM. use this relation to get the concentration of both and then using that concentration or molarity and volume which is 500ml and molar mass find the mass of weak electrolyte and its conjugate base.
(using henderson hassel bach equation)
Describe in detail how you would make 500mL of a 250mM buffer at pH 9.0 from the following
list of chemicals:
1M NaOH.
1M HCl.
Ammonium chloride salt (solid).
30% Ammonia (liquid).
For your buffer preparation give all species present and their concentrations in solution. first use henderson hasselbach equation to find the relation between weak electrolyte and its conjugate ion.The buffer concentration is 250 mM. This means that the total concentration of the weak electrolyte and its conjugate base is 100 mM. use this relation to get the concentration of both and then using that concentration or molarity and volume which is 500ml and molar mass find the mass of weak electrolyte and its conjugate base.
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