Using ClustalOmega (available here), compare the nucleotide sequence of the regulatory region for each of the reporter gene variants to the wildtype. Ensure that you keep the “>Ara..." header for each sequence as this tells the software where one sequence ends and another begins. You can simply copy and paste the nucleotide sequences provided on Moodle (including the headers) into the submission box on the Clustal website, make sure that each time you include the wildtype sequence for comparison, ensure that you select DNA from the dropdown box. For each ClustalOmega alignment, ensure that you compare the wildtype sequence with at least ONE of the mutant sequences. b) Based on your in silico analysis (as described in the accompanying video) of each of the reporter constructs, describe how the phenotype displayed in the lab might be explained through a mutation. Ensure that you justify your response by stating how the mutations would result in a change in the transcription of the reporter gene.
Using ClustalOmega (available here), compare the nucleotide sequence of the regulatory region for each of the reporter gene variants to the wildtype. Ensure that you keep the “>Ara..." header for each sequence as this tells the software where one sequence ends and another begins. You can simply copy and paste the nucleotide sequences provided on Moodle (including the headers) into the submission box on the Clustal website, make sure that each time you include the wildtype sequence for comparison, ensure that you select DNA from the dropdown box. For each ClustalOmega alignment, ensure that you compare the wildtype sequence with at least ONE of the mutant sequences. b) Based on your in silico analysis (as described in the accompanying video) of each of the reporter constructs, describe how the phenotype displayed in the lab might be explained through a mutation. Ensure that you justify your response by stating how the mutations would result in a change in the transcription of the reporter gene.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Using ClustalOmega (available here), compare the nucleotide sequence of the regulatory
region for each of the reporter gene variants to the wildtype. Ensure that you keep the
“>Ara..." header for each sequence as this tells the software where one sequence ends and
another begins. You can simply copy and paste the nucleotide sequences provided on
Moodle (including the headers) into the submission box on the Clustal website, make sure
that each time you include the wildtype sequence for comparison, ensure that you select
DNA from the dropdown box. For each ClustalOmega alignment, ensure that you compare
the wildtype sequence with at least ONE of the mutant sequences.
b) Based on your in silico analysis (as described in the accompanying video) of each of the
reporter constructs, describe how the phenotype displayed in the lab might be explained
through a mutation.
Ensure that you justify your response by stating how the mutations would result in a change
in the transcription of the reporter gene.
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