Use the graph provided to calculate the percent of DNA that remains denatured at 30 °C after cooling from 100 °C.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
4
1.5
Use the graph provided to calculate the percent
1O 1.4 +
of DNA that remains denatured at 30 °C after
cooling from 100 °C.
Cooling
1.2 +
(Hybridization)
1.1
Heating
(Denaturation)
1.0
<>
30
40
50
60
70
80
90
100
Temperature /°C
Absorbance at T
Absorbance at 30 °C
Transcribed Image Text:1.5 Use the graph provided to calculate the percent 1O 1.4 + of DNA that remains denatured at 30 °C after cooling from 100 °C. Cooling 1.2 + (Hybridization) 1.1 Heating (Denaturation) 1.0 <> 30 40 50 60 70 80 90 100 Temperature /°C Absorbance at T Absorbance at 30 °C
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