Use the Following information to answer questionse EIA results are recorded as optical-density (OD) ratios. The OD ratio is the ratio of absorbance of the tested sample to the absorbance of a control sample. The greater the OD ratio, the more "positive" is the test result. The EIA, as with most other screening tests, is not perfect; there is some overlap of optical-density ratios of samples that are actually antibody positive and those that are actually antibody negative. This is illustrated in the following figure: Hypothetical distribution of results on an EIA for HIV, by actual antibody status In terms of sensitivity and specificity, what happens if you lower the cut-off from "A" to "C"? Actualy without antbody Actualy have antbody In terms of sensitivity and specificity, what happens if you raise the cut-off from "A" to "B"? OD Rato Mumber of persons

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EIA results are recorded as optical-density (OD) ratios. The OD ratio is the ratio of absorbance of the tested sample to the
absorbance of a control sample. The greater the OD ratio, the more "positive" is the test result. The EIA, as with most other
screening tests, is not perfect; there is some overlap of optical-density ratios of samples that are actually antibody positive and
those that are actually antibody negative. This is illustrated in the following figure:
Hypothetical distribution of results on an EIA for HIV,
by actual antibody status
In terms of sensitivity and specificity, what happens if you lower
the cut-off from "A" to "C"?
Actualy
without
antibody
Actualy
In terms of sensitivity and specificity, what happens if you raise
have
antbody
the cut-off from "A" to "B"?
CA
OD Ratio
Establishing the cutoff value to define a
positive test result from a negative one is
somewhat arbitrary. You initially decide that
optical density ratios greater than "A" on
the above figure are positive.
suOGod o oqunN
Transcribed Image Text:Use the Following information to answer questionss EIA results are recorded as optical-density (OD) ratios. The OD ratio is the ratio of absorbance of the tested sample to the absorbance of a control sample. The greater the OD ratio, the more "positive" is the test result. The EIA, as with most other screening tests, is not perfect; there is some overlap of optical-density ratios of samples that are actually antibody positive and those that are actually antibody negative. This is illustrated in the following figure: Hypothetical distribution of results on an EIA for HIV, by actual antibody status In terms of sensitivity and specificity, what happens if you lower the cut-off from "A" to "C"? Actualy without antibody Actualy In terms of sensitivity and specificity, what happens if you raise have antbody the cut-off from "A" to "B"? CA OD Ratio Establishing the cutoff value to define a positive test result from a negative one is somewhat arbitrary. You initially decide that optical density ratios greater than "A" on the above figure are positive. suOGod o oqunN
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