Use the following information to answer questions 20-24: As a MLT, you are responsible for making Lysis Buffer. You need to make 1.0 L of the Lysis Buffer. The Standard Operating Procedure (SOP) in your lab states the Lysis Buffer has the final concentration of the following: 0.15M Ammonium Chloride (NH4CI) 1g of Potassium bicarbonate (KHCO3) into 1.0L solution 0.0001M Disodium EDTA (MW = 372.24 g/mol) The GWM of KHCO3 is 100.12 g/mol. Your scale is precise to the one hundredths digit. Per protocol, you measured out 1.00 gram of potassium bicarbonate (KHCO3) and mixed it in 1.0 L of the solution. What is the final concentration (in molarity, aka M) of potassium bicarbonate in this Lysis Buffer? (Enter numerical value only; no units.)

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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Use the following information to answer questions 20-24:
As a MLT, you are responsible for making Lysis Buffer. You need to make
1.0 L of the Lysis Buffer. The Standard Operating Procedure (SOP) in your
lab states the Lysis Buffer has the final concentration of the following:
0.15M Ammonium Chloride (NH4CI)
1g of Potassium bicarbonate (KHCO3) into 1.0L solution
0.0001M Disodium EDTA (MW = 372.24 g/mol)
####
TRE ARE TH
The GWM of KHCO3 is 100.12 g/mol. Your scale is precise to the one
hundredths digit. Per protocol, you measured out 1.00 gram of potassium
bicarbonate (KHCO3) and mixed it in 1.0 L of the solution.
What is the final concentration (in molarity, aka M) of potassium
bicarbonate in this Lysis Buffer? (Enter numerical value only; no units.)
Transcribed Image Text:Use the following information to answer questions 20-24: As a MLT, you are responsible for making Lysis Buffer. You need to make 1.0 L of the Lysis Buffer. The Standard Operating Procedure (SOP) in your lab states the Lysis Buffer has the final concentration of the following: 0.15M Ammonium Chloride (NH4CI) 1g of Potassium bicarbonate (KHCO3) into 1.0L solution 0.0001M Disodium EDTA (MW = 372.24 g/mol) #### TRE ARE TH The GWM of KHCO3 is 100.12 g/mol. Your scale is precise to the one hundredths digit. Per protocol, you measured out 1.00 gram of potassium bicarbonate (KHCO3) and mixed it in 1.0 L of the solution. What is the final concentration (in molarity, aka M) of potassium bicarbonate in this Lysis Buffer? (Enter numerical value only; no units.)
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