Use the data in the table below to plot a standard curve (this must be submitted with the problem set) and determine the concentration of the unknowns if possible (Graph paper from the back of the lab manual must be used to create the standard curve). If it is not possible to determine the concentration of the unknown(s), state why and what you would need to do (explain in detail) in order to determine the concentration of the unknown(s). If the concentration of the unknown(s) can be determined, clearly illustrate this on the graph and clearly explain how you determined the concentration of the unknown(s).       SAMPLE PROTEIN  CONCENTRATION ABSORBANCE,  610 nm Standard 1 1 mg/ml 0.064 Standard 2 2.5 mg/ml 0.154 Standard 3 5 mg/ml 0.304 Standard 4 7.5 mg/ml 0.449 Standard 5 10 mg/ml 0.613 Unknown A   0.850 Unknown B   0.475

Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter14: Chemical Equilibrium
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Use the data in the table below to plot a standard curve (this must be submitted with the problem set) and determine the concentration of the unknowns if possible (Graph paper from the back of the lab manual must be used to create the standard curve). If it is not possible to determine the concentration of the unknown(s), state why and what you would need to do (explain in detail) in order to determine the concentration of the unknown(s). If the concentration of the unknown(s) can be determined, clearly illustrate this on the graph and clearly explain how you determined the concentration of the unknown(s).

 

 

 

SAMPLE

PROTEIN  CONCENTRATION

ABSORBANCE,

 610 nm

Standard 1

1 mg/ml

0.064

Standard 2

2.5 mg/ml

0.154

Standard 3

5 mg/ml

0.304

Standard 4

7.5 mg/ml

0.449

Standard 5

10 mg/ml

0.613

Unknown A

 

0.850

Unknown B

 

0.475

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