Use the data in the table below to plot a standard curve (this must be submitted with the problem set) and determine the concentration of the unknowns if possible (Graph paper from the back of the lab manual must be used to create the standard curve). If it is not possible to determine the concentration of the unknown(s), state why and what you would need to do (explain in detail) in order to determine the concentration of the unknown(s). If the concentration of the unknown(s) can be determined, clearly illustrate this on the graph and clearly explain how you determined the concentration of the unknown(s). SAMPLE PROTEIN CONCENTRATION ABSORBANCE, 610 nm Standard 1 1 mg/ml 0.064 Standard 2 2.5 mg/ml 0.154 Standard 3 5 mg/ml 0.304 Standard 4 7.5 mg/ml 0.449 Standard 5 10 mg/ml 0.613 Unknown A 0.850 Unknown B 0.475
Use the data in the table below to plot a standard curve (this must be submitted with the problem set) and determine the concentration of the unknowns if possible (Graph paper from the back of the lab manual must be used to create the standard curve). If it is not possible to determine the concentration of the unknown(s), state why and what you would need to do (explain in detail) in order to determine the concentration of the unknown(s). If the concentration of the unknown(s) can be determined, clearly illustrate this on the graph and clearly explain how you determined the concentration of the unknown(s). SAMPLE PROTEIN CONCENTRATION ABSORBANCE, 610 nm Standard 1 1 mg/ml 0.064 Standard 2 2.5 mg/ml 0.154 Standard 3 5 mg/ml 0.304 Standard 4 7.5 mg/ml 0.449 Standard 5 10 mg/ml 0.613 Unknown A 0.850 Unknown B 0.475
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
Use the data in the table below to plot a standard curve (this must be submitted with the problem set) and determine the concentration of the unknowns if possible (Graph paper from the back of the lab manual must be used to create the standard curve). If it is not possible to determine the concentration of the unknown(s), state why and what you would need to do (explain in detail) in order to determine the concentration of the unknown(s). If the concentration of the unknown(s) can be determined, clearly illustrate this on the graph and clearly explain how you determined the concentration of the unknown(s).
|
SAMPLE |
PROTEIN CONCENTRATION |
ABSORBANCE,610 nm |
|
Standard 1 |
1 mg/ml |
0.064 |
|
Standard 2 |
2.5 mg/ml |
0.154 |
|
Standard 3 |
5 mg/ml |
0.304 |
|
Standard 4 |
7.5 mg/ml |
0.449 |
|
Standard 5 |
10 mg/ml |
0.613 |
|
Unknown A |
|
0.850 |
|
Unknown B |
|
0.475 |
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