Transfer of Culture from Agar Plate to Agar Slant
____ a.) With your free hand, pick up the sterile nutrient agar slant tube. Remove the cap by grasping the cap with the little finger of the hand that is holding the loop.
____ b.) Raise the lid of a petri plate sufficiently to access a colony with your sterile loop. Pick up the organism.
____ c.) Clean your work area with disinfectant. Allow area to dry.
____ d.) Flame an inoculating loop until it is red-hot. Allow the loop to cool.
____ e.) Remove the loop from the tube and flame the mouth of the tube. Replace the cap on the tube.
____ f.) After transfer is done, flame the loop.
____ g.) Incubate the nutrient agar slant at 37°C for 24–48 hours.
____ h.) Flame the mouth of the tube and insert the loop into the tube to inoculate the surface of the slant, using a zigzag motion.
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