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- Which of the following will decrease the limit of resolution (Select all that applies) a) using lower wavelength of illumination b) using an objective lens with lower numerical aperature c) using immersion oil d) using electron as the source of illumination instead of light6. Moves stage up and down for focusing; large amounts a) Fine Focus Knob b) Large Focus Knob c) Course Pycus Knob d) Small Fotus Knobi) Explain why the crystalline lens of the eye gets a virtual object from the cornea.
- 3. Which term refers to the clarity of the image? (a) magnification, (b) resolution, (c) contrastCompute the total magnification. [Use 10x magnification for eyepiece]1. Scanning2. LPO3. HPO4. OIOContrast how the views differ under high power (40x) and oil immerson (100x) objective lenses in terms of a) magnification b) detail of the cell, c) size of the area being viewed and d) resolution.
- It is desirable that the microscope objectives be parfocal because the specimen will be .................. a)blurry due to the changes in the refractive index of the medium b)stay in focus when the light is turned off c)have more contrast and enhanced details d)remain in focus if the magnification is changed e)none of the answer choicesHow will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityD) When performing fluorescence microscopy what are the stokes shift and why is it better to have fluorochromes with a large stokes shift? E) What is photobleaching and what is done when imaging histological samples to overcome it when performing fluorescence microscopy?
- What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece, Revolving Nosepiece, Objective Lens, Stage and Condenser? Explain each1) In the amniote eye, light rays are bent by the greatest amount when they pass from the _______." A) cornea into the lens B) air into the cornea C) aqueous humor into the lens D) front of the lens to the back of the lens 2) When a light ray passes into lens at a more perpendicular angle _____ then when it passes into the lens at a more oblique angle. A) it undergoes more bending B) it undergoes less bending C) the amount of bending the light ray undergoes is independent of the angle at which it hits the lensMy daughter have eye retina area is yellow. Please suggest biotechnological solution.Describe your methodology.