To prevent any signs of contaminants in the broth it is sterilized by heating. Sterilization by heating is an important aspect of the experiment, but it is not the major factor that influences the result.
To prevent any signs of contaminants in the broth it is sterilized by heating. Sterilization by heating is an important aspect of the experiment, but it is not the major factor that influences the result.
A broth refers to the growing media for microorganisms. It consists of ingredients that are known to influence the growth of the organisms. For example, glucose, peptone and water. Contamination in microbiology is defined as the growth of any unwanted microorganism in the culture media. The entry of a contaminant is always accidental and unintended in the media. Contaminants can be bacteria, yeasts, moulds.
The sterilization of the broth typically takes place in an autoclave. The autoclave is an instrument involving steam sterilization at high temperature and pressure. The pressure in an autoclave reaches 15 pounds per square inch and the highest temperature is 121℃.
A broth after preparation is always sterilized to eliminate any chances of contamination while making the media.
Any component of the media can be contaminated. Thus sterilization after forming the broth ensures safety during microbial culture.
Microbial contamination can result from the glasswares, air or the hands while preparing the media. Thus it is mandatory to sterilize a broth for microorganism culture.
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