This is an example of a very positive ONPO ONPG test Your result

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question

What does this indicate? Is ONPG positive or negative

**ONPG Test**

The ONPG test is used to detect the presence of the enzyme β-galactosidase, which cleaves the substrate ONPG (o-nitrophenyl-β-D-galactopyranoside) to produce a yellow compound, o-nitrophenol.

**Images:**

1. **Positive ONPG Test Example:**
   - The first image shows a test tube containing a bright yellow liquid, indicative of a very positive ONPG test result. This coloration suggests a high activity of β-galactosidase, meaning the organism can effectively hydrolyze the ONPG substrate.

2. **Test Result:**
   - The second image displays a similar test tube with a yellow liquid. The label "Your result" implies this is an example of the observer's specific test outcome. The presence of yellow coloration suggests a positive reaction, confirming some level of β-galactosidase activity.

Understanding these results can help in biochemical analyses to determine the metabolic capabilities of microorganisms.
Transcribed Image Text:**ONPG Test** The ONPG test is used to detect the presence of the enzyme β-galactosidase, which cleaves the substrate ONPG (o-nitrophenyl-β-D-galactopyranoside) to produce a yellow compound, o-nitrophenol. **Images:** 1. **Positive ONPG Test Example:** - The first image shows a test tube containing a bright yellow liquid, indicative of a very positive ONPG test result. This coloration suggests a high activity of β-galactosidase, meaning the organism can effectively hydrolyze the ONPG substrate. 2. **Test Result:** - The second image displays a similar test tube with a yellow liquid. The label "Your result" implies this is an example of the observer's specific test outcome. The presence of yellow coloration suggests a positive reaction, confirming some level of β-galactosidase activity. Understanding these results can help in biochemical analyses to determine the metabolic capabilities of microorganisms.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Organ system
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education