There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure out which baby goes with each set of parents. You started to look at the repeat sequence Q2. of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can have different numbers of repeats (i.e. “n" shown below can vary). 5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3' 3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5' a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of each primer's sequence) which you would use for PCR reaction to amplify the repeat sequence. Make sure to label the ends (5' and 3' of each primer). You obtain the following results. markers dad#1 mom#1 dad#2 mom#2 dad#3 mom#3 BabyA BabyB BabyC n = 50 n = 45 n = 40 n = 35 n = 30 n = 25 n = 20 | | b) Why is it that having more repeats leads to a band that is higher in the gel? c) Why is it that some people show only one band? d) Why is it that some people show two bands? e) Given the data so far, for which of the three babies (only Baby A, only Baby B, or only Baby C, all of them, or none of them) can you already conclusively tell who the parents are? ) If you amplify a DNA sequence through PCR, what are the 4 reaction components that you would absolutely need? Provide names only.
There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure out which baby goes with each set of parents. You started to look at the repeat sequence Q2. of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can have different numbers of repeats (i.e. “n" shown below can vary). 5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3' 3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5' a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of each primer's sequence) which you would use for PCR reaction to amplify the repeat sequence. Make sure to label the ends (5' and 3' of each primer). You obtain the following results. markers dad#1 mom#1 dad#2 mom#2 dad#3 mom#3 BabyA BabyB BabyC n = 50 n = 45 n = 40 n = 35 n = 30 n = 25 n = 20 | | b) Why is it that having more repeats leads to a band that is higher in the gel? c) Why is it that some people show only one band? d) Why is it that some people show two bands? e) Given the data so far, for which of the three babies (only Baby A, only Baby B, or only Baby C, all of them, or none of them) can you already conclusively tell who the parents are? ) If you amplify a DNA sequence through PCR, what are the 4 reaction components that you would absolutely need? Provide names only.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Anatomy and Physiology of Special Sensory Organs
Sensory organs can be labeled as special sensory structures that permit sight, hearing, odor, and flavor. Sensory structures permitting proprioception, touch, thermal, and pain perception can be classified as more advanced sensory organs. The sensory neurons are trained to find out modifications in the external and internal conditions so that a person's body can react to that change. A stimulus is the first signal that is recognized by any sensory receptor of the body. Stimulus is an impulse generated when there is a change in the surroundings of a person. For example, a heated environment will alert the brain through the thermal sensory organs and generate a reflex accordingly.
Sensory Receptors
The human sensory system is one of the most complex and highly evolved structures, which processes a myriad of incoming messages. This well-coordinated system helps an organism or individual to respond to external stimuli, appropriately. The sensory receptors are an important part of the sensory system. These receptors are specialized epidermal cells that respond to external environmental stimuli. These receptors consist of structural and support cells that form the peripheral unit of the receptor and the neural dendrites which receive and detect the external stimuli.
Question
please only answer parts d and e and f
Transcribed Image Text:There are three babies (Baby A, Baby B and Baby C) in a maternity ward,
and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are
Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure
out which baby goes with each set of parents. You started to look at the repeat sequence
Q2.
of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can
have different numbers of repeats (i.e. “n" shown below can vary).
5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3'
3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5'
a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of
each primer's sequence) which you would use for PCR reaction to amplify the
repeat sequence. Make sure to label the ends (5' and 3' of each primer).
You obtain the following results.
markers
dad#1
mom#1
dad#2
mom#2
dad#3
mom#3
BabyA BabyB
BabyC
n = 50
n = 45
n = 40
n = 35
n = 30
n = 25
n = 20
| |
Transcribed Image Text:b) Why is it that having more repeats leads to a band that is higher in the
gel?
c) Why is it that some people show only one band?
d) Why is it that some people show two bands?
e) Given the data so far, for which of the three babies (only Baby A, only Baby B,
or only Baby C, all of them, or none of them) can you already conclusively tell
who the parents are?
) If you amplify a DNA sequence through PCR, what are the 4 reaction
components that you would absolutely need? Provide names only.
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