Answer part a **Theme 5: Cellular Expression Patterns of Tumor Suppressor Transcription Factor p53**The provided data from Jimenez et al. (2022) illustrate the oscillating expression patterns of the tumor suppressor transcription factor p53, which is commonly mutated in cancers. The study focused on a mutated human cell line.**Panel D: Changes in p53 mRNA Levels**- The graph in Panel D shows the changes in p53 mRNA levels over time using two measurement approaches: RNA sequencing (RNASeq) and quantitative PCR (qPCR).- The x-axis represents time in hours, from 0 to 9.- The y-axis on the left corresponds to fold change (FC) in p53 mRNA relative to the baseline using qPCR, ranging from 0.5 to 1.5.- The y-axis on the right indicates RNASeq fold change, from 0.5 to 1.5 as well.- The data depict slight variations in mRNA levels over time, with overlapping error bars suggesting consistency between the two methods.**Panel E: Protein Level Measurements**- Panel E displays protein level changes, measured using Mass Spectrometry (MassSpec) and Western Blot (WB).- The x-axis is similarly marked for time in hours.- The y-axis on the left shows fold change in protein levels measured by WB, spanning from 1 to 5.- The y-axis on the right indicates MassSpec FC, ranging from 1 to 4.- This panel shows more pronounced fluctuations in protein levels compared to mRNA, with peaks observed at 3 and 5 hours.**Explanation Proposal**A) The magnitude of protein changes can be larger than the changes in mRNA due to several potential factors, including:1. **Post-transcriptional Modifications**: These can cause significant changes in protein levels and activities without corresponding mRNA changes.2. **Translation Efficiency**: Variability in how efficiently mRNA is translated into protein can amplify protein level changes.3. **Protein Stability**: Differences in protein degradation rates can also affect protein levels independently of mRNA.Understanding these differences is crucial in cellular biology, especially in the context of cancer research.

Name: Answer part a **Theme 5: Cellular Expression Patterns of Tumor Suppres
Uploaded: 2024-03-20T06:42:41.000Z
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Description: Answer part a **Theme 5: Cellular Expression Patterns of Tumor Suppressor Transcription Factor p53**The provided data from Jimenez et al. (2022) illustrate the oscillating expression patterns of the tumor suppressor transcription factor p53, which is