The vector below was used in the cloning process. Eco01091 2674 BstAPI 179 Aatll 2617 Ndel 183 Sspl 2501 Ehel 235 Xmnl 2294 Begl 2215 Scal 2177. 146 236 MCS lacz 2486 469 PUC18/19 2686 bp Sapl 683 B52 Afll, BspLU11 806 Gsul 1784, Cfr101 1779. 1626 1466 rep (PMB1) \Aw N 1217 Eco311 1766, Eam11051 1694 Explain your results when observations as stated below were obtained after the plasmids were transformed into the host cells: (i) No colonies were growing on the LB plate added with ampicillin and X-gal. (ii) Only blue colonies were growing on the LB plate added with ampicillin and X-gal. 111) Only white colonies were growing on the LB plate added with ampicillin and X-gal. bla (Ap)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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iii

The vector below was used in the cloning process.
BstAPI 179
Eco01091 2674
Aatll 2617
Sspl 2501
Ndel 183
Ehel 235
Xmnl 2294
Bcgl 2215
Scal 2177,
146/
lacz
2486
MCS
236
469
PUC18/19
2686 bp
Sapl 683
852
Afll, BspLU111 B06
Gsul 1784,
1626
Cfr101 1779.
1466
rep (PMB1)
\AWNI 1217
Eco311 1766.
Eam11051 1694.
Explain your results when observations as stated below were obtained after the plasmids were
transformed into the host cells:
(i)
No colonies were growing on the LB plate added with ampicillin and X-gal.
(ii)
Only blue colonies were growing on the LB plate added with ampicillin and X-gal.
(iii)
Only white colonies were growing on the LB plate added with ampicillin and X-gal.
bla (Ap)
Transcribed Image Text:The vector below was used in the cloning process. BstAPI 179 Eco01091 2674 Aatll 2617 Sspl 2501 Ndel 183 Ehel 235 Xmnl 2294 Bcgl 2215 Scal 2177, 146/ lacz 2486 MCS 236 469 PUC18/19 2686 bp Sapl 683 852 Afll, BspLU111 B06 Gsul 1784, 1626 Cfr101 1779. 1466 rep (PMB1) \AWNI 1217 Eco311 1766. Eam11051 1694. Explain your results when observations as stated below were obtained after the plasmids were transformed into the host cells: (i) No colonies were growing on the LB plate added with ampicillin and X-gal. (ii) Only blue colonies were growing on the LB plate added with ampicillin and X-gal. (iii) Only white colonies were growing on the LB plate added with ampicillin and X-gal. bla (Ap)
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