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The Unknown specie for identification is Staphylococcus epidermidis

The Unknown specie for identification is Staphylococcus epidermidis

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The Unknown specie for identification is Staphylococcus epidermidis
**Creating a Flow Chart or Dichotomous Key for Bacterial Identification** --- **Instructions:** make a flow chart or a dichotomous key indicating, step by step, how you arrived at your identification. For example: - Why you selected specific tests and stains. - How you interpreted the results of those tests and stains. - Why you accepted or did not accept a specific test result. **Sample Bacteria List:** 1. Bacillus subtilis 2. Escherichia coli 3. Klebsiella pneumoniae 4. Micrococcus luteus 5. Proteus mirabilis 6. Pseudomonas aeruginosa 7. Serratia marcescens 8. Staphylococcus aureus 9. Staphylococcus epidermidis 10. Streptococcus agalactiae **Steps to Identify Bacteria:** 1. **Initial Stain Selection:** - **Gram Stain**: This is done to differentiate between Gram-positive and Gram-negative bacteria. (Explains the cell wall structure differences) - **Result Interpretation**: - Gram-positive: Purple color. - Gram-negative: Pink color. 2. **Further Testing (Gram-positive vs. Gram-negative):** - For **Gram-positive**: - **Catalase Test**: - Catalase positive: Presence of bubbles (e.g., Staphylococci). - Catalase negative: No bubbles (e.g., Streptococci). - If Catalase positive, proceed with: - **Coagulase Test**: - Coagulase positive: Clumping (e.g., Staphylococcus aureus). - Coagulase negative: No clumping (e.g., Staphylococcus epidermidis). - If Catalase negative: - **Hemolysis on Blood Agar**: - Beta-hemolysis: Clear zone (e.g., Streptococcus agalactiae). - Gamma-hemolysis: No hemolysis. - For **Gram-negative**: - **Oxidase Test**: - Oxidase positive: Blue/purple color change (e.g., Pseudomonas aeruginosa). - Oxidase negative: No color change (e.g., others). - **Lactose Fermentation (MacConkey Agar)**: - Lactose fermenter:
Transcribed Image Text:**Creating a Flow Chart or Dichotomous Key for Bacterial Identification** --- **Instructions:** make a flow chart or a dichotomous key indicating, step by step, how you arrived at your identification. For example: - Why you selected specific tests and stains. - How you interpreted the results of those tests and stains. - Why you accepted or did not accept a specific test result. **Sample Bacteria List:** 1. Bacillus subtilis 2. Escherichia coli 3. Klebsiella pneumoniae 4. Micrococcus luteus 5. Proteus mirabilis 6. Pseudomonas aeruginosa 7. Serratia marcescens 8. Staphylococcus aureus 9. Staphylococcus epidermidis 10. Streptococcus agalactiae **Steps to Identify Bacteria:** 1. **Initial Stain Selection:** - **Gram Stain**: This is done to differentiate between Gram-positive and Gram-negative bacteria. (Explains the cell wall structure differences) - **Result Interpretation**: - Gram-positive: Purple color. - Gram-negative: Pink color. 2. **Further Testing (Gram-positive vs. Gram-negative):** - For **Gram-positive**: - **Catalase Test**: - Catalase positive: Presence of bubbles (e.g., Staphylococci). - Catalase negative: No bubbles (e.g., Streptococci). - If Catalase positive, proceed with: - **Coagulase Test**: - Coagulase positive: Clumping (e.g., Staphylococcus aureus). - Coagulase negative: No clumping (e.g., Staphylococcus epidermidis). - If Catalase negative: - **Hemolysis on Blood Agar**: - Beta-hemolysis: Clear zone (e.g., Streptococcus agalactiae). - Gamma-hemolysis: No hemolysis. - For **Gram-negative**: - **Oxidase Test**: - Oxidase positive: Blue/purple color change (e.g., Pseudomonas aeruginosa). - Oxidase negative: No color change (e.g., others). - **Lactose Fermentation (MacConkey Agar)**: - Lactose fermenter:
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