Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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The selenium content in soil can be determined fluorometrically upon conversion of
the selenium to the fluorescent product shown, followed by extraction in cyclohexane.
Emission from the fluorescent product is observed at 518 nm.
a
The selenium content in a 0.147 g soil sample was converted to the fluorescent product
and extracted in 50.0 mL of cyclohexane. The method of standard additions was used to determine the selenium content in the
soil sample. Initially, 3.00 mL of the cyclohexane solution were placed in a cuvette for fluorescence determination. Several small
additions of a 2.66 µg Se/mL standard solution containing the fluorescent product were added to the 3.00 mL solution in the
cuvette. The fluorescence was measured at 518 nm. The results are shown in the table.
Total added Se standard
volume (µl)
0.00
20.0
40.0
60.0
80.0
Se wt% =
50625
Fluorescence intensity
(arbitrary units)
Determine the weight percent (wt%) of selenium in the soil sample and find the absolute uncertainty in the weight percent of
selenium. Report the weight percent and absolute uncertainty with three significant figures.
Incorrect
54.31
64.5
76.7
91.1
108.3
.0021
Se
Incorrect
88"
Transcribed Image Text:Macmillan Learning
The selenium content in soil can be determined fluorometrically upon conversion of
the selenium to the fluorescent product shown, followed by extraction in cyclohexane.
Emission from the fluorescent product is observed at 518 nm.
a
The selenium content in a 0.147 g soil sample was converted to the fluorescent product
and extracted in 50.0 mL of cyclohexane. The method of standard additions was used to determine the selenium content in the
soil sample. Initially, 3.00 mL of the cyclohexane solution were placed in a cuvette for fluorescence determination. Several small
additions of a 2.66 µg Se/mL standard solution containing the fluorescent product were added to the 3.00 mL solution in the
cuvette. The fluorescence was measured at 518 nm. The results are shown in the table.
Total added Se standard
volume (µl)
0.00
20.0
40.0
60.0
80.0
Se wt% =
50625
Fluorescence intensity
(arbitrary units)
Determine the weight percent (wt%) of selenium in the soil sample and find the absolute uncertainty in the weight percent of
selenium. Report the weight percent and absolute uncertainty with three significant figures.
Incorrect
54.31
64.5
76.7
91.1
108.3
.0021
Se
Incorrect
88
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