The restriction enzymes KpnI and Acc65I recognize and cleave the same 6 bp sequence. However, the sticky end formed from KpnI cleavage cannot be ligated directly to the sticky end formed from Acc651 cleavage. ↓ 5' 'GG TACC 3' 3' CCATGG 5' ↑ Kpnl What could explain this? 5' GGTACC 3' 3' C CAT GG 5' Acc65l Each restriction enzyme breaks different bonds within the 6 bp sequence. Sticky ends can never be joined without a chemically synthesized DNA linker. One or both restrictions enzymes do not produce an overhang at the cleavage site. One of the restriction enzymes removes the sticky end created by the other enzyme.
The restriction enzymes KpnI and Acc65I recognize and cleave the same 6 bp sequence. However, the sticky end formed from KpnI cleavage cannot be ligated directly to the sticky end formed from Acc651 cleavage. ↓ 5' 'GG TACC 3' 3' CCATGG 5' ↑ Kpnl What could explain this? 5' GGTACC 3' 3' C CAT GG 5' Acc65l Each restriction enzyme breaks different bonds within the 6 bp sequence. Sticky ends can never be joined without a chemically synthesized DNA linker. One or both restrictions enzymes do not produce an overhang at the cleavage site. One of the restriction enzymes removes the sticky end created by the other enzyme.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Concept explainers
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Topic Video
Question
b
Transcribed Image Text:The restriction enzymes KpnI and Acc65I recognize and cleave the same 6 bp sequence. However, the sticky end formed from
KpnI cleavage cannot be ligated directly to the sticky end formed from Acc651 cleavage.
5' GGTACC 3'
3' C CAT GG 5'
↑
Kpnl
What could explain this?
5' GGTACC 3'
3' C CATGG 5'
Acc651
Each restriction enzyme breaks different bonds within the 6 bp sequence.
Sticky ends can never be joined without a chemically synthesized DNA linker.
One or both restrictions enzymes do not produce an overhang at the cleavage site.
One of the restriction enzymes removes the sticky end created by the other enzyme.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education