The range (smallest to largest size) of the ladder is _____ ### Western Blot AnalysisThis image shows a Western blot analysis used to identify protein bands corresponding to the heavy and light chains of an antibody.#### Components:- **Lane 1**: Molecular weight markers represented in kilodaltons (kDa) on the left side for reference, including markers at 3, 6, 14, 18, 28, 38, 49, 62, and 188 kDa.- **Lane 2**: Protein sample lane containing the antibody. Distinct bands are visible in this lane. #### Bands:- **Heavy Chain Band**: A prominent band appears around 49 kDa, labeled as the "heavy chain."- **Light Chain Band**: A second band appears around 28 kDa, labeled as the "light chain."#### Interpretation:In this analysis, proteins are separated based on molecular weight. The presence of bands indicates successful detection of specific protein chains of the antibody, with the molecular weight of the heavy chain being approximately 49 kDa and the light chain being approximately 28 kDa.This technique is commonly used to verify the presence and integrity of proteins in research and diagnostic applications.

Biochemistry is the science of study of the internal biochemical processes of organisms. The…

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Science

Biochemistry

The range (smallest to largest size) of the ladder is _____

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

The range (smallest to largest size) of the ladder is _____

### Western Blot Analysis

This image shows a Western blot analysis used to identify protein bands corresponding to the heavy and light chains of an antibody.

#### Components:

- **Lane 1**: Molecular weight markers represented in kilodaltons (kDa) on the left side for reference, including markers at 3, 6, 14, 18, 28, 38, 49, 62, and 188 kDa.
- **Lane 2**: Protein sample lane containing the antibody. Distinct bands are visible in this lane.

#### Bands:

- **Heavy Chain Band**: A prominent band appears around 49 kDa, labeled as the "heavy chain."
- **Light Chain Band**: A second band appears around 28 kDa, labeled as the "light chain."

#### Interpretation:

In this analysis, proteins are separated based on molecular weight. The presence of bands indicates successful detection of specific protein chains of the antibody, with the molecular weight of the heavy chain being approximately 49 kDa and the light chain being approximately 28 kDa.

This technique is commonly used to verify the presence and integrity of proteins in research and diagnostic applications.

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