help #13 The peptide Leu-Cys-Arg-Ser-Gln-Met is subjected to Edman degradation. In the first cycle the peptide first reacts withphenylisothiocyanate under basic conditions. The product of this reaction is incubated with anhydrous trifluoroacetic acid andsubsequently with an aqueous acid. What are the products generated in the first cycle.PTH-Leu, PTH-Cys, PTH-Arg, PTH-Ser, PTH-Gln, and PTH-MetPTH-Leu-Cys-Arg-Ser-Gln-MetPTH-Met and Leu-Cys-Arg-Ser-Gln-MetPTH-Leu-Cys and PTH-Arg-Ser-Gln-MetPTH-Leu and Cys-Arg-Ser-Gln-Met

Step 1:In the first step of Edman degradation, the amino acid at the beginning (N-terminal) of the…

Answered: The peptide Leu-Cys-Arg-Ser-Gln-Met is…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

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Author:Michael Cummings

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Chapter10: From Proteins To Phenotypes

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Problem 14QP: If phenylalanine was not an essential amino acid, would diet therapy (the elimination of...

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The peptide Leu-Cys-Arg-Ser-Gln-Met is subjected to Edman degradation. After the first cycle of this process, the products that are generated are: O PTH-Leu, PTH Cys, PTH-Arg. PTH-Ser, PTH-GIn, and PTH-Met O PTH-Leu-Cys-Arg-Ser-Gin-Met O PTH-Met and Leu-Cys-Arg-Ser-Gin-Met O PTH-Leu-Cys and PTH-Arg-Ser-Gin-Met O PTH-Leu and Cys-Arg-Ser-Gln-Met
The purinosome contains enzymes that convert the serine hydroxymethylgroup to the formyl group of 10-formyltetrahydrofolate. Write a balancedequation for each reaction in this conversion.
Azaserine inhibits amidotransferases. State the purine precursor that resembles azaserine. Which step in the purine biosynthetic pathway will be inhibited?
Radioactive uracil can be used to label all of the pyrimidine residues inDNA. Using either names or structures, present pathways for the conversionof uracil to dTTP and to dCTP. For each reaction, show the involvement ofcofactors, and identify sites of allosteric regulation. Similarly, hypoxanthine (HX) can be used to label purine residues. As given above, write reactions showing the conversion of hypoxanthine to dATP and dGTP.
Cells defective in thymidylate synthase are able to undergo cell division if methotrexate and thymidine are both provided; however, cells with fully functional thymidylate synthase cannot divide under these same culture conditions. What is the biochemical basis for this observation?
Explain the chemical change that occurs in converting kynurenine (a product of tryptophan degradation) to kynurenate, a reaction in which α-ketoglutarate is transformed to glutamate.
In the pyrimidine degradative pathway, all pyrimidines undergo conversion to uracil, which undergoes an NADPH-dependent reduction. Show plausible reactions leading from cytidine, cytosine, and uridine to uracil, and write a plausible structure for the product of uracil reduction (dihydrouracil).
A seven-residue peptide was found to have an amino acid composition including glu, val, Lys, met, leu, and trp (there is more than one copy of one amino acid). Several reactions on samples of the peptide resulted in the following: a) Trypsin has no effect on the heptapeptide. b) If one cycle of Edman degradation provides the PTH derivative shown below, what does this tell you about the oligopeptide under investigation? [image] c) Chymotrypsin treatment yields a dipeptide, a tetrapeptide, and a free amino acid. Possibilities? d) The tetrapeptide contains val, lys, and met (only). What information does this give you? e) Cyanogen bromide treatment generates a dipeptide, a tetrapeptide, and free Lys. f)What is the sequence of the peptide?
Write a balanced equation for the synthesis of TMP from dUMP that is coupled to the conversion of serine into glycine.
Describe the chemical difference between mannose and heptyl alpha-D-mannose. Why does FimH have a higher affinity for heptyl a-D-mannose versus methyl a-D-mannose?
The sucrose substitute tagatose is produced by hydrolyzing lactose and then chemically converting one of the two resulting aldoses to a ketose. Which residue of lactose gives rise to tagatose?
A mutant form of polypeptide hormone angiotensin II has the amino acid composition (Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val) The following observations are made: Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest. • Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others. • Chymotrypsin cleaves the hormone into two tetrapeptides, of composition (Asp, Arg, Tyr, Val) and (Ile, Met, Phe, Pro) • The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase. What is the sequence of angiotensin II?

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