The observed fluorescence lifetime in the absence of a quencher is 1.4 ns. In the presence of a quencher the fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the f luorescence quantum yieldsin the presence and absence of the quencher. φF/φF,0.

The observed fluorescence lifetime in the absence of a quencher is 1.4 ns. In the presence of a quencher the fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the f luorescence quantum yieldsin the presence and absence of the quencher. φF/φF,0.

Inquiry into Physics

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Chapter10: Atomic Physics

Section: Chapter Questions

Problem 35Q

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The observed fluorescence lifetime in the absence of a quencher is 1.4 ns. In the presence of a quencher the fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the f luorescence quantum yields
in the presence and absence of the quencher. φ_F/φ_F,0.

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