The MPN mostly looks to grow E. coli as the indicator species of fecal contamination. Most E. coli is not pathogenic, and you can get many more harmful things like viruses from fecal contamination. Why is E. coli the only thing we look for in the lab to determine whether water is contaminated with fecal matter? (like why not look for the viruses?)
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The MPN mostly looks to grow E. coli as the indicator species of fecal contamination. Most E. coli is not pathogenic, and you can get many more harmful things like viruses from fecal contamination. Why is E. coli the only thing we look for in the lab to determine whether water is contaminated with fecal matter? (like why not look for the viruses?)
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- As you can see on the blood agar plates, some microbes can lyse red blood cells releasing nutrients into the surrounding area. What might the advantage be for a bacteria to be able to do this? Do you think this could have an effect on the bacteria’s virulence? Explain.Hepatitis B Virus is 42nm in diameter. Sacromyces cervisiae (a yeast) has a diameter of 4um in length. Staphylococcus aureus (bacteria) are 0.001mm in diameter. Put the three microbes in order from smallest to largest. Hepatitis B, S. cervisiae, S. aureus Hepatitis B, S. aureus, S. cervisiae, S. aureus, Hepatitis B, S. cervisiae, S. cervisiae, Hepatitis B, S. aureus S. cervisiae, S. aureus, Hepatitis B, S. aureus, S. cervisiae, Hepatitis BNote that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! ThanksThe world is facing a Corona Virus (COVID-19) Pandemic and as a potential scientist and a microbiology student, you have been required to prepare the easiest and most affordable hand sanitizer that can be utilized by students in various microbiology laboratories. Each student in your class has been given 96% ethanol and you are required to prepare 70% ethanol hand sanitizer in a 2L spray bottle. Describe clearly the method you will use in order to achieve the aim of the practical.
- You work for the U.S Department of Agriculture's Agricultural Marketing Services, which is responsible for the microbiological testing of foods that are purchased for federal nutritional programs such as the National School Lunch Program. Specifically, your job involves monitoring the total bacterial counts as well as the E. Coli counts in ground beef using standard plate count techniques. The figure below shows the procedure you used and the results you obtained after testing a sample of ground beef. 2. What was the concentration of E. Coli (CFU/gram) in the ground beef sample? SHOW ALL WORK AND USE SCIENTIFIC NOTATION WHEN WRITING YOUR ANSWER.(c) Public health scientists wanted to investigate the effect of ionising radiation on the growth of Salmonella typhimurium in the presence of antibiotics. They grew bacterial colonies of Salmonella typhimurium in petri dishes labelled A, B and C. The Petri dishes were kept at 36°C for 48 hours, with scientists observing the bacteria every 24 hours. The results for this investigation are below. Petri dish A B с Treatment Was subjected to ionising radiation followed immediately by a dose of antibiotic X An equal amount of antibiotic X was added (as was added to Petri dish A) Was given neither ionising radiation nor antibiotic X After 24 hours Small spots of growth on surface No growth Growth across whole surface After 48 hours (ii) Identify the purpose of petri dish C. Large spots of growth on surface No growth Growth across whole surface (i) Explain the effect of the ionising radiation on the bacteria. (iii) Explain why scientists must continue to develop new antibiotics.The microbiology department is celebrating the end of the school year in May by holding its traditional picnic on the green. The speeches drag on for a couple of hours, but finally all the faculty and students can dig into the food: chicken salad, tomatoes, onions, salad, and custard pie. By evening, the whole department, except for two vegetarian students who did not eat the chicken salad, is stricken with nausea, vomiting, retching, and abdominal cramping. Several individuals complain of diarrhea. One patient shows signs of shock (low blood pressure). Blood and stool samples are collected from patients, and an analysis of all foods served at the meal is conducted. Bacteria can cause gastroenteritis (inflammation of the stomach and intestinal tract) either by colonizing and replicating in the host, which is considered an infection, or by secreting toxins, which is considered intoxication. Signs and symptoms of infections are typically delayed, whereas intoxication manifests within…
- As part of your job in an international pharmaceutical company you are given the task of: a) Developing a cheaper method for the production of vitamin C b) Developing a live cholera vaccine Describe how you would accomplish these tasksSome bacteria produce the enzyme penicillinase, which breaks the beta-lactam ring in the penicillin molecule and converts it into penicilloic acid, which is harmless to bacteria. This is an example of which of the following mechanisms of antibiotic resistance? which option below is the answer? Destruction or inactivation of the antibiotic by the microbe Preventing penetration to the target site within the microbe Alteration of the drug’s target site(s) within the microbe None of the other four answers are correct Rapid ejection (pumping) of the drug out of the microbial cellWhich of the following cannot be grown in artificial media and must be grown in live animals or cells instead? Neisseria gonorrhoeae, the causative agent of gonorrhea Lactococcus lactis, a lactic acid bacterium used to produce cheddar cheese Staphylococcus aureus, a bacterium sometimes found in human nares (nostrils) Staphylococcus epidermidis, a bacterium routinely found on human skin Escherichia coli, a bacterium common in mammalian intestines 21 Treponema pallidum, the causative agent of syphilis