The mini-prep silica column will preferentially bind deoxyribonucleotides. How is it that at the enc of our prep we're left with ONLY plasmid DNA and not a mix of plasmid & chromosomal DNA? The chromosomal DNA was precipitated during neutralization Bacterial chromosomes are methylated so don't stick to silica Chromosomal DNA is too large to bind to the column O We do, but the amount of chromosomal DNA is small enough to not matter too much

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The mini-prep silica column will preferentially bind deoxyribonucleotides. How is it that at the enc
of our prep we're left with ONLY plasmid DNA and not a mix of plasmid & chromosomal DNA?
The chromosomal DNA was precipitated during neutralization
Bacterial chromosomes are methylated so don't stick to silica
Chromosomal DNA is too large to bind to the column
O We do, but the amount of chromosomal DNA is small enough to not matter too much
Question 4
We started a miniprep with a completely opaque culture indicating the cells grew well. We finish
our miniprep (not noticing anything out of the ordinary) and discover that the DNA concentration is
10ug/ml, quite low. Which of the following might explain this result best?
Our Lysis was inefficient
Our elution buffer was expired
O We forgot to add antibiotic to our O/N cultures
"Our spectrophotometer is likely broken
Transcribed Image Text:The mini-prep silica column will preferentially bind deoxyribonucleotides. How is it that at the enc of our prep we're left with ONLY plasmid DNA and not a mix of plasmid & chromosomal DNA? The chromosomal DNA was precipitated during neutralization Bacterial chromosomes are methylated so don't stick to silica Chromosomal DNA is too large to bind to the column O We do, but the amount of chromosomal DNA is small enough to not matter too much Question 4 We started a miniprep with a completely opaque culture indicating the cells grew well. We finish our miniprep (not noticing anything out of the ordinary) and discover that the DNA concentration is 10ug/ml, quite low. Which of the following might explain this result best? Our Lysis was inefficient Our elution buffer was expired O We forgot to add antibiotic to our O/N cultures "Our spectrophotometer is likely broken
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