The migration distances r, for each of the proteln standards bands and that of the unknown protein have been measured in the table below. The migration distance of the tracking dye has been measured to be ro- 6.3 cm. Protein ru (cm) M, (Da) Myosin 0.4 200,000 8-galactosidase 1.5 116,250 Glycogen phosphorylase b 1.9 97,400 Bovine serum albumin 2.5 66,200 Ovalbumin 3.4 45,000 Carbonic anhydrase 4.3 31,000 Soybean trypsin inhibitor 5.1 21,500 Lysozyme 5.6 14,400 Unknown protein 3.9 TBD Use the calibration curve shown to the right of the figure in question 3.1 as an example to reproduce this curve for the current data set. Copy the data in the table above into an Excel spreadsheet and pay special attention to the parameters you should plot on the y- and x-axes. Hint: additional calculation is required in Excel. If your calculation is correct, you should be able to get a linear correlation, If your correlation is not linear, this is an indication that you have done something wrong. Please go back to the graph and make sure you have made the appropriate calculations. Once you obtain the linear correlation, write the linear equation of that correlation in the format: yrax+b in which you would express "a" and "b" to 2 decimal places with the proper sign "+" or "-" Please do not include any spaces in your answer.
The migration distances r, for each of the proteln standards bands and that of the unknown protein have been measured in the table below. The migration distance of the tracking dye has been measured to be ro- 6.3 cm. Protein ru (cm) M, (Da) Myosin 0.4 200,000 8-galactosidase 1.5 116,250 Glycogen phosphorylase b 1.9 97,400 Bovine serum albumin 2.5 66,200 Ovalbumin 3.4 45,000 Carbonic anhydrase 4.3 31,000 Soybean trypsin inhibitor 5.1 21,500 Lysozyme 5.6 14,400 Unknown protein 3.9 TBD Use the calibration curve shown to the right of the figure in question 3.1 as an example to reproduce this curve for the current data set. Copy the data in the table above into an Excel spreadsheet and pay special attention to the parameters you should plot on the y- and x-axes. Hint: additional calculation is required in Excel. If your calculation is correct, you should be able to get a linear correlation, If your correlation is not linear, this is an indication that you have done something wrong. Please go back to the graph and make sure you have made the appropriate calculations. Once you obtain the linear correlation, write the linear equation of that correlation in the format: yrax+b in which you would express "a" and "b" to 2 decimal places with the proper sign "+" or "-" Please do not include any spaces in your answer.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
Related questions
Question
6
Transcribed Image Text:The migration distances r, for each of the protein standards bands and that of the unknown
protein have been measured in the table below. The migration distance of the tracking dye has
been measured to be ro = 6.3 cm.
Protein
ru (cm) M, (Da)
Myosin
0.4
200,000
B-galactosidase
1.5
116,250
Glycogen phosphorylase b 1.9
97,400
Bovine serum albumin
2.5
66,200
Ovalbumin
3.4
45,000
Carbonic anhydrase
4.3
31,000
Soybean trypsin inhibitor 5.1
21,500
Lysozyme
5.6
14,400
Unknown protein
3.9
TBD
Use the calibration curve shown to the right of the figure in question 3.1 as an example to
reproduce this curve for the current data set. Copy the data in the table above into an Excel
spreadsheet and pay special attention to the parameters you should plot on the y- and x-axes. Hint:
additional calculation is required in Excel. If your calculation is correct, you should be able to get a
linear correlation, If your correlation is not linear, this is an indication that you have done
something wrong. Please go back to the graph and make sure you have made the appropriate
calculations.
Once you obtain the linear correlation, write the linear equation of that correlation in the format:
y-ax+b in which you would express "a" and "b" to 2 decimal places with the proper sign "+".
Please do not include any spaces in your answer.
or "-"
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 2 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY