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The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. 10.0 M of Nat. 5.0 M of Nat. c. 0.1 M of Nat. d. 1.0 M of Nat. e. None of the above. b.

The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. 10.0 M of Nat. 5.0 M of Nat. c. 0.1 M of Nat. d. 1.0 M of Nat. e. None of the above. b.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. b. c. d. e. 10.0 M of Nat. 5.0 M of Nat. 0.1 M of Nat. 1.0 M of Nat. None of the above.
Transcribed Image Text:The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. b. c. d. e. 10.0 M of Nat. 5.0 M of Nat. 0.1 M of Nat. 1.0 M of Nat. None of the above.
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