The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. 10.0 M of Nat. 5.0 M of Nat. c. 0.1 M of Nat. d. 1.0 M of Nat. e. None of the above. b.
The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. 10.0 M of Nat. 5.0 M of Nat. c. 0.1 M of Nat. d. 1.0 M of Nat. e. None of the above. b.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![The melting temperature (Tm) of 500 bases long DNA is described by the equation:
Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a
conditional term of the equation meaning that it applies in a certain range of C
concentrations.
What is a concentration of C+ (sodium ion) commonly used in hybridization?
a.
b.
c.
d.
e.
10.0 M of Nat.
5.0 M of Nat.
0.1 M of Nat.
1.0 M of Nat.
None of the above.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F321fd000-515a-4cf5-ac03-99096af58e6a%2Fdf7723fa-6421-4f4c-bf85-caf1bea63e13%2F0d8j10h_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The melting temperature (Tm) of 500 bases long DNA is described by the equation:
Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a
conditional term of the equation meaning that it applies in a certain range of C
concentrations.
What is a concentration of C+ (sodium ion) commonly used in hybridization?
a.
b.
c.
d.
e.
10.0 M of Nat.
5.0 M of Nat.
0.1 M of Nat.
1.0 M of Nat.
None of the above.
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