The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. 10.0 M of Nat. 5.0 M of Nat. c. 0.1 M of Nat. d. 1.0 M of Nat. e. None of the above. b.

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Chapter1: Biochemistry: An Evolving Science
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The melting temperature (Tm) of 500 bases long DNA is described by the equation:
Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a
conditional term of the equation meaning that it applies in a certain range of C
concentrations.
What is a concentration of C+ (sodium ion) commonly used in hybridization?
a.
b.
c.
d.
e.
10.0 M of Nat.
5.0 M of Nat.
0.1 M of Nat.
1.0 M of Nat.
None of the above.
Transcribed Image Text:The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %) + 16.6 log [C]. In this equation term: 16.6 log [C] is a conditional term of the equation meaning that it applies in a certain range of C concentrations. What is a concentration of C+ (sodium ion) commonly used in hybridization? a. b. c. d. e. 10.0 M of Nat. 5.0 M of Nat. 0.1 M of Nat. 1.0 M of Nat. None of the above.
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