The measurement of alanine aminotransferase activity (reaction rate) usually includes an excess of pure lactate dehydrogenase and NADH in the reaction system. The rate of alanine disappearance is equal to the rate of NADH disappearance measured spectrophotometrically. How does this assay work?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
100%

Answer this question 

O Macmillan Learning
The measurement of alanine aminotransferase activity (reaction rate) usually includes an excess of pure lactate dehydrogenase
and NADH in the reaction system. The rate of alanine disappearance is equal to the rate of NADH disappearance
measured
spectrophotometrically.
How does this assay work?
NADH is the substrate for alanine synthesis. Alanine aminotransferase metabolizes alanine at the same rate as
alanine synthesis.
O Alanine and NADH are substrates for alanine aminotransferase. The reaction equilibrium favors alanine and lactate
dehydrogenase is added to remove the product.
O Alanine aminotransferase and lactate dehydrogenase metabolize alanine and NADH at the same rate, respectively.
Alanine aminotransferase requires the cofactor NADH to metabolize alanine.
Alanine aminotransferase catalyzes the rate-limiting reaction in a coupled-reaction assay. Lactate dehydrogenase
rapidly metabolizes the pyruvate product, using NADH, as quickly as it forms.
Transcribed Image Text:O Macmillan Learning The measurement of alanine aminotransferase activity (reaction rate) usually includes an excess of pure lactate dehydrogenase and NADH in the reaction system. The rate of alanine disappearance is equal to the rate of NADH disappearance measured spectrophotometrically. How does this assay work? NADH is the substrate for alanine synthesis. Alanine aminotransferase metabolizes alanine at the same rate as alanine synthesis. O Alanine and NADH are substrates for alanine aminotransferase. The reaction equilibrium favors alanine and lactate dehydrogenase is added to remove the product. O Alanine aminotransferase and lactate dehydrogenase metabolize alanine and NADH at the same rate, respectively. Alanine aminotransferase requires the cofactor NADH to metabolize alanine. Alanine aminotransferase catalyzes the rate-limiting reaction in a coupled-reaction assay. Lactate dehydrogenase rapidly metabolizes the pyruvate product, using NADH, as quickly as it forms.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON