The Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate concentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vmax and Km.
The Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate concentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vmax and Km.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Q8:
![The Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate
concentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum
rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the
slope in terms of the constants Vmax and Km.
Incorrect
-1/Km
x-intercept
Lineweaver-Burk Plot
1/v
v-intercept
Vmax
Slope
Km/Vmax
1/[S]
1/Km
-Km/Vma
1/Vmax
-Vmax/Km
Answer Bank
-Vmax
Km
-Km
-1/Vmax
Vmax/Km](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc94cb5d1-997a-49d0-b0ef-9ec02bf0a187%2F53d59573-30e3-4025-a793-dadc3a089e63%2Fro2rofe_processed.png&w=3840&q=75)
Transcribed Image Text:The Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate
concentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum
rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the
slope in terms of the constants Vmax and Km.
Incorrect
-1/Km
x-intercept
Lineweaver-Burk Plot
1/v
v-intercept
Vmax
Slope
Km/Vmax
1/[S]
1/Km
-Km/Vma
1/Vmax
-Vmax/Km
Answer Bank
-Vmax
Km
-Km
-1/Vmax
Vmax/Km
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