The isoelectric point (pl) of a peptide is the pH at which the Group pK peptide ceases to migrate in an electric field. Since a peptide is aspartate 3.7 zwitterionic, there are the same number of positive charges as glutamate 4.3 negative charges on the peptide population. The pl can be histidine 6.0 estimated within 0.1 or 0.2 pH units from the pK values of all cysteine 8.2 the proton dissociable groups in the peptide. tyrosine 10.1 Determine the total positive charge on the peptide HANSEN lysine 10.5 when all acidic and basic groups are fully protonated. Enter arginine 12.5 your answer without the sign. peptide amino group 8.0 peptide carboxyl group 3.4 charge: Select the pK values used to estimate the pl. O 3.4 O 4.3 O 6.0 O 8.0 Estimate the pl value of the HANSEN peptide. pl =

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.