The following non-coding sequence (i.e. template strand) is transcribed by the RNA pol II into a pre-mRNA. 5'-CTAACTGCTATGCAT-3' a) Determine the mRNA synthesized by the RNA pol II. You have to write the sequence from the 5' end to the 3' end and indicate those ends as shown in the original sequence. b) Using the genetic code provided below, determine the amino acid sequence if the mRNA determined in part a) were translated. You have to write the amino acid sequence from the N-terminus end to the C-terminus end and indicate those at each and in parentheses. For example: (N)-.......-(C) A mutation occurred in the nucleotide sequence, and the new non-coding sequence is shown below: 5'-CTAACTGCTAGTGCAT-3' c) Indicate the specific mutation that occurred in the non-coding strand and its type. d) What consequence will the mutation have on the polypeptide chain? Justify your answer and write the new polypeptide sequence if it has changed as a result of this mutation. You have to write the amino acid sequence from the N-terminus end to the C-terminus end and indicate those at each and in parentheses. For example: (N)-.......-(C)

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Chapter1: The Human Body: An Orientation
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First base in codon
The following non-coding sequence (i.e. template strand) is transcribed by the RNA pol II into
a pre-mRNA.
5'-CTAACTGCTATGCAT-3'
a) Determine the mRNA synthesized by the RNA pol II.
You have to write the sequence from the 5' end to the 3' end and indicate those ends as shown
in the original sequence.
b) Using the genetic code provided below, determine the amino acid sequence if the mRNA
determined in part a) were translated.
You have to write the amino acid sequence from the N-terminus end to the C-terminus end and
indicate those at each and in parentheses. For example: (N)-.......-(C)
A mutation occurred in the nucleotide sequence, and the new non-coding sequence is shown
below: 5'-CTAACTGCTAGTGCAT-3'
c) Indicate the specific mutation that occurred in the non-coding strand and its type.
d) What consequence will the mutation have on the polypeptide chain? Justify your answer
and write the new polypeptide sequence if it has changed as a result of this mutation.
You have to write the amino acid sequence from the N-terminus end to the C-terminus end and
indicate those at each and in parentheses. For example: (N)-.......-(C)
U
C
A
G
UUU
UUC-
UUA
UUG
U
UCU
UCC
UCA
UCG
CUU
CCU
CUC
CCC
CUA
CCA
CUG
CCG
AUU
ACU
AUC Ile (1)
ACC
AUA
ACA
AUG Met (M) start ACG-
GUU-
GCU
GUC
GCC
GUA
GCA
GUG-
GCG
Phe (F)
Leu (L)
Leu (L)
Second base in codon
Val (V)
с
Ser (S)
Pro (P)
Thr (T)
Ala (A)
UAU
UAC-
UAA
UAG
CAU
CAC
CAA 1
CAG
AAU
AAC
AAA 1
AAG
A
GAU
GAC
GAA 1
GAG
Tyr (Y)
STOP
His (H)
Gin (Q)
Asn (N)
Lys (K)
Asp (D)
Glu (E)
G
UGU
UGC
UGA STOP
UGG Trp (W)
CGU
CGC
CGA
CGG
AGU
AGC
AGA
AGG
GGU
GGC
GGA
GGG
Cys (C)
Arg (R)
Ser (S)
Arg (R)
Gly (G)
U
C
A
G
U
C
A
G
DUAGUAG
C
C
А
Last base
codon
Transcribed Image Text:First base in codon The following non-coding sequence (i.e. template strand) is transcribed by the RNA pol II into a pre-mRNA. 5'-CTAACTGCTATGCAT-3' a) Determine the mRNA synthesized by the RNA pol II. You have to write the sequence from the 5' end to the 3' end and indicate those ends as shown in the original sequence. b) Using the genetic code provided below, determine the amino acid sequence if the mRNA determined in part a) were translated. You have to write the amino acid sequence from the N-terminus end to the C-terminus end and indicate those at each and in parentheses. For example: (N)-.......-(C) A mutation occurred in the nucleotide sequence, and the new non-coding sequence is shown below: 5'-CTAACTGCTAGTGCAT-3' c) Indicate the specific mutation that occurred in the non-coding strand and its type. d) What consequence will the mutation have on the polypeptide chain? Justify your answer and write the new polypeptide sequence if it has changed as a result of this mutation. You have to write the amino acid sequence from the N-terminus end to the C-terminus end and indicate those at each and in parentheses. For example: (N)-.......-(C) U C A G UUU UUC- UUA UUG U UCU UCC UCA UCG CUU CCU CUC CCC CUA CCA CUG CCG AUU ACU AUC Ile (1) ACC AUA ACA AUG Met (M) start ACG- GUU- GCU GUC GCC GUA GCA GUG- GCG Phe (F) Leu (L) Leu (L) Second base in codon Val (V) с Ser (S) Pro (P) Thr (T) Ala (A) UAU UAC- UAA UAG CAU CAC CAA 1 CAG AAU AAC AAA 1 AAG A GAU GAC GAA 1 GAG Tyr (Y) STOP His (H) Gin (Q) Asn (N) Lys (K) Asp (D) Glu (E) G UGU UGC UGA STOP UGG Trp (W) CGU CGC CGA CGG AGU AGC AGA AGG GGU GGC GGA GGG Cys (C) Arg (R) Ser (S) Arg (R) Gly (G) U C A G U C A G DUAGUAG C C А Last base codon
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