The enzyme cholesterol oxidase catalyzes the reaction between cholesterol and oxygen to form cholest-4-ene-3-one and hydrogen peroxide. Explain how you could use this enzyme to make a voltammetric electrode that produces a current that changes with changing cholesterol concentration

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Q: Determine ∆G° for a reaction when ∆G = -88.9 kJ/mol and Q = 0.043 at 298 K. (R = 8.314 J/mol ･ K)

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Potentiometric Titrations

Potentiometric titration is one of the important titration methods and is mainly carried out to characterize acid solutions. It follows a method similar to that of the normal titration technique, but the main difference is that there is no indicator used in the potentiometric titrations. Instead of using an indicator, electrodes are employed for monitoring the whole titration process. A scientist named Robert Behrend did the potentiometric titration for the first time, and it was done using the mercurous solution and potassium chloride.