The concentration of Sn*+ can be determined fluorometrically upon chelation with flavonol in a solution of sulfuric acid and dimethylforamide (DMF). The Sn*-flavonol complex produces fluorescence in the blue region of the spectrum, which can be quantitatively determined with a fluorometer. The method of standard additions was used to determine the concentration of Sn+ in an unknown solution. To begin, 5.00 mL aliquots of the unknown solution were added to five, separate 25.0 mL volumetric flasks. To each volumetric flask, a different amount of a standard 2.41 ug/mL Sn+ solution was added. The added volumes of the standard Sn+ solution were 0.00, 2.00, 5.00, 8.00, and 10.00 mL. Then, to each volumetric flask, 5.00 mL of DMF, 3.00 mL of 1M sulfuric acid, and an excess amount of flavonol was added. The flasks were diluted to 25.0 mL with deionized water and inverted several times to thoroughly mix. The flasks were left standing for 15 min to ensure chelation, and the fluorescence intensity was measured with a fluorometer. The results of the experiment are shown in the table. Sn+ standard volume Sample volume (mL) Fluorescence (mL) intensity 5.00 0.00 14.45 5.00 2.00 17.27 5.00 5.00 21.30 5.00 8.00 26.25 5.00 10.00 28.35 Construct a standard addition plot from the given data. Determine the slope and its standard deviation for the plot you have created. 1.418 ml- 0.041 mL- Incorrect Determine the y-intercept and its standard deviation for the plot you have created. 14.43 0.25
The concentration of Sn*+ can be determined fluorometrically upon chelation with flavonol in a solution of sulfuric acid and dimethylforamide (DMF). The Sn*-flavonol complex produces fluorescence in the blue region of the spectrum, which can be quantitatively determined with a fluorometer. The method of standard additions was used to determine the concentration of Sn+ in an unknown solution. To begin, 5.00 mL aliquots of the unknown solution were added to five, separate 25.0 mL volumetric flasks. To each volumetric flask, a different amount of a standard 2.41 ug/mL Sn+ solution was added. The added volumes of the standard Sn+ solution were 0.00, 2.00, 5.00, 8.00, and 10.00 mL. Then, to each volumetric flask, 5.00 mL of DMF, 3.00 mL of 1M sulfuric acid, and an excess amount of flavonol was added. The flasks were diluted to 25.0 mL with deionized water and inverted several times to thoroughly mix. The flasks were left standing for 15 min to ensure chelation, and the fluorescence intensity was measured with a fluorometer. The results of the experiment are shown in the table. Sn+ standard volume Sample volume (mL) Fluorescence (mL) intensity 5.00 0.00 14.45 5.00 2.00 17.27 5.00 5.00 21.30 5.00 8.00 26.25 5.00 10.00 28.35 Construct a standard addition plot from the given data. Determine the slope and its standard deviation for the plot you have created. 1.418 ml- 0.041 mL- Incorrect Determine the y-intercept and its standard deviation for the plot you have created. 14.43 0.25
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![The concentration of Sn*+ can be determined fluorometrically upon chelation with flavonol in a solution of sulfuric acid and
dimethylforamide (DMF). The Sn*-flavonol complex produces fluorescence in the blue region of the spectrum, which can be
quantitatively determined with a fluorometer.
The method of standard additions was used to determine the concentration of Sn+ in an unknown solution. To begin, 5.00 mL
aliquots of the unknown solution were added to five, separate 25.0 mL volumetric flasks. To each volumetric flask, a different
amount of a standard 2.41 pg/mL Sn** solution was added. The added volumes of the standard Sn+ solution were 0.00, 2.00,
5.00, 8.00, and 10.00 mL. Then, to each volumetric flask, 5.00 mL of DMF, 3.00 mL of 1M sulfuric acid, and an excess amount
of flavonol was added. The flasks were diluted to 25.0 mL with deionized water and inverted several times to thoroughly mix.
The flasks were left standing for 15 min to ensure chelation, and the fluorescence intensity was measured with a fluorometer. The
results of the experiment are shown in the table.
Sn+ standard volume
Sample volume
(mL)
Fluorescence
(mL)
intensity
5.00
0.00
14.45
5.00
2.00
17.27
5.00
5.00
21.30
5.00
8.00
26.25
5.00
10.00
28.35
Construct a standard addition plot from the given data. Determine the slope and its standard deviation for the plot you
have created.
m 3=
1.418
mL-1
0.041
mL-
Incorrect
Determine the y-intercept and its standard deviation for the plot you have created.
b 3=
14.43
0.25
Incorrect
Determine the concentration and Sn+ and its standard deviation in the unknown solution.
5.576
0.188
(Sn+] =
Hg/mL
Hg/mL
Incorrect
Incorrect](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8d84af8e-a113-41a8-9492-584c8d22bd6f%2Fbc857365-3479-430f-807b-d21e04717cba%2Fqs31ej_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The concentration of Sn*+ can be determined fluorometrically upon chelation with flavonol in a solution of sulfuric acid and
dimethylforamide (DMF). The Sn*-flavonol complex produces fluorescence in the blue region of the spectrum, which can be
quantitatively determined with a fluorometer.
The method of standard additions was used to determine the concentration of Sn+ in an unknown solution. To begin, 5.00 mL
aliquots of the unknown solution were added to five, separate 25.0 mL volumetric flasks. To each volumetric flask, a different
amount of a standard 2.41 pg/mL Sn** solution was added. The added volumes of the standard Sn+ solution were 0.00, 2.00,
5.00, 8.00, and 10.00 mL. Then, to each volumetric flask, 5.00 mL of DMF, 3.00 mL of 1M sulfuric acid, and an excess amount
of flavonol was added. The flasks were diluted to 25.0 mL with deionized water and inverted several times to thoroughly mix.
The flasks were left standing for 15 min to ensure chelation, and the fluorescence intensity was measured with a fluorometer. The
results of the experiment are shown in the table.
Sn+ standard volume
Sample volume
(mL)
Fluorescence
(mL)
intensity
5.00
0.00
14.45
5.00
2.00
17.27
5.00
5.00
21.30
5.00
8.00
26.25
5.00
10.00
28.35
Construct a standard addition plot from the given data. Determine the slope and its standard deviation for the plot you
have created.
m 3=
1.418
mL-1
0.041
mL-
Incorrect
Determine the y-intercept and its standard deviation for the plot you have created.
b 3=
14.43
0.25
Incorrect
Determine the concentration and Sn+ and its standard deviation in the unknown solution.
5.576
0.188
(Sn+] =
Hg/mL
Hg/mL
Incorrect
Incorrect
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