The ability to produce proteins through genetic engineering of microbial and mam- malian cells and the need for high purity therapeutic proteins has established a need for efficient large scale protein purification schemes. The system of Continuous Affinity-Recycle Extraction (CARE) combines the advantages of well accepted separation methods, e.g. affinity chromatography, liquid extraction and membrane filtration, while avoiding the drawbacks inherent in batch and column operations. The technical feasibility of the CARE system was studied using B-galactosidase affinity purification as a test system. Figure P12.5 shows the process. What is the recy- cle flow rate in mL/hr in each stream? Assume that the concentrations of U are equiva lent to the concentrations of the B-galactosidase in solution, and that steady state exists.
The ability to produce proteins through genetic engineering of microbial and mam- malian cells and the need for high purity therapeutic proteins has established a need for efficient large scale protein purification schemes. The system of Continuous Affinity-Recycle Extraction (CARE) combines the advantages of well accepted separation methods, e.g. affinity chromatography, liquid extraction and membrane filtration, while avoiding the drawbacks inherent in batch and column operations. The technical feasibility of the CARE system was studied using B-galactosidase affinity purification as a test system. Figure P12.5 shows the process. What is the recy- cle flow rate in mL/hr in each stream? Assume that the concentrations of U are equiva lent to the concentrations of the B-galactosidase in solution, and that steady state exists.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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The ability to produce proteins through genetic engineering of microbial and mam- malian cells and the need for high purity therapeutic proteins has established a need for efficient large scale protein purification schemes.
The system of Continuous Affinity-Recycle Extraction (CARE) combines the advantages of well accepted separation methods, e.g. affinity chromatography, liquid extraction and membrane filtration, while avoiding the drawbacks inherent in batch and column operations.
The technical feasibility of the CARE system was studied using B-galactosidase affinity purification as a test system. Figure P12.5 shows the process. What is the recy- cle flow rate in mL/hr in each stream? Assume that the concentrations of U are equiva lent to the concentrations of the B-galactosidase in solution, and that steady state exists.
Transcribed Image Text:Desorbing
Buffer
60 mLh
O U/mL
Feed
600 mLh
1.37 U/mL
19.3 U/ml
ABSORBING
STAGE
DESORBING
STAGE
Recycle
5.2 U/ml
600 mL/h
60 mLh
Waste
Product
0.08 U/mL
12.9 U/mL
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