TABLE 5.3 The sequence specificities of some proteolytic enzymes and cyanogen bromide R, O R2 O N-terminal -..-N- C-...C-teminal нн Enzyme Preferred Site Source R, = Lys, Arg From digestive systems of animals, many other sources Trypsin Chymotrypsin R, = Tyr, Trp, Phe, Leu Same as trypsin R, = Arg From blood; involved in coagulation Thrombin R, = Asp, Glu V-8 protease Prolyl endopeptidase Subtilisin From Staphylococcus aureus = Pro Lamb kidney, other tissues Very little specificity From various bacilli Carboxypeptidase A R2 = C-terminal amino acid From digestive systems of animals Thermolysin R2 = Leu, Val, Ile, Met From Bacillus thermoproteolyticus Cyanogen bromide R1 = Met

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Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.