Table 2. Starter plate conditions. Starter Plate Bacterial Cas9 DNA Repair System SGRNA Donor Plate Additives Colony Color Template DNA IX IPTG, X-gal Blue OFF IX/ARA IPTG, X-gal, Blue ON arabinose A. Using evidence from Table 2, explain in complete sentences why the bacterial colonies on the starter plates are blue. B. If the bacteria on the starter plates did NOT have a functional lacZ gene, what color would you expect the colonies to be? C. Explain how the differences between the IX and IXIARA starter plates may influence gene editing in the laboratory activity.
Table 2. Starter plate conditions. Starter Plate Bacterial Cas9 DNA Repair System SGRNA Donor Plate Additives Colony Color Template DNA IX IPTG, X-gal Blue OFF IX/ARA IPTG, X-gal, Blue ON arabinose A. Using evidence from Table 2, explain in complete sentences why the bacterial colonies on the starter plates are blue. B. If the bacteria on the starter plates did NOT have a functional lacZ gene, what color would you expect the colonies to be? C. Explain how the differences between the IX and IXIARA starter plates may influence gene editing in the laboratory activity.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Table 2. Starter plate conditions.
Starter
Plate
Bacterial
Cas9
DNA Repair
System
SGRNA
Donor
Plate
Additives
Colony Color
Template DNA
IX
IPTG, X-gal
Blue
OFF
IX/ARA
IPTG, X-gal,
Blue
ON
arabinose
A. Using evidence from Table 2, explain in complete sentences why the bacterial colonies on
the starter plates are blue.
B. If the bacteria on the starter plates did NOT have a functional lacZ gene, what color would
you expect the colonies to be?
C. Explain how the differences between the IX and IXIARA starter plates may influence gene
editing in the laboratory activity.
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