Table 1. PCR Components Final Concentration Volume for 1x Volume Initial PCR Components Concentration [C1] for 13x [C2] [V1] [V2] sterile distilled H20 PCR Buffer Magnesium Chloride (MgCl2) DNTP Mix 10x 1x 50 mM 1.5 mM 10 mM 0.4 mM 10 µM 10 μΜ 5U/µL 100 ng/µL 0.3 μΜ 0.3 µM 10/25 µL 100 ng Forward Primers Reverse Primers Taq Polymerase genomic DNA template Total 25.0µL
Table 1. PCR Components Final Concentration Volume for 1x Volume Initial PCR Components Concentration [C1] for 13x [C2] [V1] [V2] sterile distilled H20 PCR Buffer Magnesium Chloride (MgCl2) DNTP Mix 10x 1x 50 mM 1.5 mM 10 mM 0.4 mM 10 µM 10 μΜ 5U/µL 100 ng/µL 0.3 μΜ 0.3 µM 10/25 µL 100 ng Forward Primers Reverse Primers Taq Polymerase genomic DNA template Total 25.0µL
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Answer the table below:
![28
Table 1. PCR Components
Volume
Final
Concentration
Volume
for 1x
Initial
PCR Components
for 13x
Concentration [C1]
[C2]
[V1]
[V2]
sterile distilled H20
PCR Buffer
Magnesium
Chloride (MgCl2)
DNTP Mix
10x
1x
50 mM
1.5 mM
0.4 mM
0.3 µM
0.3 μΜ
10/25 µL
100 ng
10 mM
Forward Primers
Reverse Primers
Taq Polymerase
genomic DNA
template
10 µM
10 μΜ.
5U/µL
100 ng/µL
Total
25.0µL](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F02b3581b-0061-4e54-8785-0d7cb8002a4f%2F9a18fd9d-e68b-4f15-8244-a2d88353dca0%2Fiodza8e_processed.png&w=3840&q=75)
Transcribed Image Text:28
Table 1. PCR Components
Volume
Final
Concentration
Volume
for 1x
Initial
PCR Components
for 13x
Concentration [C1]
[C2]
[V1]
[V2]
sterile distilled H20
PCR Buffer
Magnesium
Chloride (MgCl2)
DNTP Mix
10x
1x
50 mM
1.5 mM
0.4 mM
0.3 µM
0.3 μΜ
10/25 µL
100 ng
10 mM
Forward Primers
Reverse Primers
Taq Polymerase
genomic DNA
template
10 µM
10 μΜ.
5U/µL
100 ng/µL
Total
25.0µL
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