- Switch to the low-power (10x) objective and notice that the depth of field is shallower. If you constantly use the focusing knob, the three-dimensional form of the threads or hair can be determined. (question 1, 2)   What happens to the depth of focus when magnification increased?

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- Switch to the low-power (10x) objective and notice that the depth of field is shallower. If you constantly use the focusing knob, the three-dimensional form of the threads or hair can be determined. (question 1, 2)

 

  1. What happens to the depth of focus when magnification increased?

 

2 .Discuss planes of focus and depth of field based upon your observations of both the scanning and low-power objectives. Note: The plane of focus is a specific distance from the lens where the specimen can be sharply focused and each microscope lens has a restrictive plane of focus.

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