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Suppose you want to test the results of a transformation by growing E. coli cells in LB medium containing ampicillin as the antibiotic for selection. Ampicillin at a concentration of 100 µg/mL will kill cells that do not contain an ampicillin resistance gene, but will allow the growth of cells that have been transformed with this gene. The concentrated stock of ampicillin is 100 mg/mL. How many microliters of the ampicillin stock should you add to 250 mL of LB for a bacterial culture? ATPase proteins hydrolyze ATP to ADP + Pi. In order to check the ATPase activity of a chaperone, one can perform an ATPase assay. The assay is performed in a tube with a 200 μL total reaction volume, containing ATP at a concentration of 2 mM. The stock solution of ATP is 150 mM in buffer. What volume of the stock solution do you need to add to the reaction mixture to make it 2 mM ATP? How much ATP (in solid form) do you need to weigh, in order to make 1.5 mL of a stock solution which has a concentration of 150 mM ATP? The molecular weight of ATP is 551.14 (according to the label on the bottle of powder you found on the chemical shelf).

Suppose you want to test the results of a transformation by growing E. coli cells in LB medium containing ampicillin as the antibiotic for selection. Ampicillin at a concentration of 100 µg/mL will kill cells that do not contain an ampicillin resistance gene, but will allow the growth of cells that have been transformed with this gene. The concentrated stock of ampicillin is 100 mg/mL. How many microliters of the ampicillin stock should you add to 250 mL of LB for a bacterial culture? ATPase proteins hydrolyze ATP to ADP + Pi. In order to check the ATPase activity of a chaperone, one can perform an ATPase assay. The assay is performed in a tube with a 200 μL total reaction volume, containing ATP at a concentration of 2 mM. The stock solution of ATP is 150 mM in buffer. What volume of the stock solution do you need to add to the reaction mixture to make it 2 mM ATP? How much ATP (in solid form) do you need to weigh, in order to make 1.5 mL of a stock solution which has a concentration of 150 mM ATP? The molecular weight of ATP is 551.14 (according to the label on the bottle of powder you found on the chemical shelf).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Suppose you want to test the results of a transformation by growing E. coli cells in LB medium containing ampicillin as the antibiotic for selection. Ampicillin at a concentration of 100 µg/mL will kill cells that do not contain an ampicillin resistance gene, but will allow the growth of cells that have been transformed with this gene. The concentrated stock of ampicillin is 100 mg/mL. How many microliters of the ampicillin stock should you add to 250 mL of LB for a bacterial culture? ATPase proteins hydrolyze ATP to ADP + Pi. In order to check the ATPase activity of a chaperone, one can perform an ATPase assay. The assay is performed in a tube with a 200 µL total reaction volume, containing ATP at a concentration of 2 mM. The stock solution of ATP is 150 mM in buffer. What volume of the stock solution do you need to add to the reaction mixture to make it 2 mM ATP? How much ATP (in solid form) do you need to weigh, in order to make 1.5 mL of a stock solution which has a concentration of 150 mM ATP? The molecular weight of ATP is 551.14 (according to the label on the bottle of powder you found on the chemical shelf).
Transcribed Image Text:Suppose you want to test the results of a transformation by growing E. coli cells in LB medium containing ampicillin as the antibiotic for selection. Ampicillin at a concentration of 100 µg/mL will kill cells that do not contain an ampicillin resistance gene, but will allow the growth of cells that have been transformed with this gene. The concentrated stock of ampicillin is 100 mg/mL. How many microliters of the ampicillin stock should you add to 250 mL of LB for a bacterial culture? ATPase proteins hydrolyze ATP to ADP + Pi. In order to check the ATPase activity of a chaperone, one can perform an ATPase assay. The assay is performed in a tube with a 200 µL total reaction volume, containing ATP at a concentration of 2 mM. The stock solution of ATP is 150 mM in buffer. What volume of the stock solution do you need to add to the reaction mixture to make it 2 mM ATP? How much ATP (in solid form) do you need to weigh, in order to make 1.5 mL of a stock solution which has a concentration of 150 mM ATP? The molecular weight of ATP is 551.14 (according to the label on the bottle of powder you found on the chemical shelf).
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