spectrophotometric determination of chlorophyll-a concentration in Spinach: Should we force the trendline for the plot of absorbance versus concentration through the origin. Why or why not? And if we added (0 0) to your data set is that the same and should we do it why or why not?
spectrophotometric determination of chlorophyll-a concentration in Spinach: Should we force the trendline for the plot of absorbance versus concentration through the origin. Why or why not? And if we added (0 0) to your data set is that the same and should we do it why or why not?
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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spectrophotometric determination of chlorophyll-a concentration in Spinach:
Should we force the trendline for the plot of absorbance versus concentration through the origin. Why or why not? And if we added (0 0) to your data set is that the same and should we do it why or why not?
Transcribed Image Text:Absorbance vs. Concentration
2.5
2
1.5
y = 3114.4x + 0.7991
R = 0.9958
0.5
0.
0.00E+00
1.00E-04
2.00E-04
3.00E-04
4.00 E-04
5.00E-04
Сопсеntration
Absorbance
Expert Solution
Initials
The above mentioned figure is called calibration curve.
It is an important tool in UV spectroscopy.
Rather it is means by which one can determine concentration in unknown sample.
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