Solvent Front 0 H Spotting Line 2. Which one is the more polar component? Explain

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Which one is the more polar component? Explain.

1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than
component B. The following result is obtained. Calculate the Rf for each component and label the identity of each
spot. 2
Solvent Front
0
0
Spotting Line
2. Which one is the more polar component? Explain
3. If a more polar solvent (mobil phase) is used for the above experiment, the result will be:
a) Both component A and Component B will travel further up the plate
b) Only Component A will travel further up the plat
c) Only Component B will travel further up the plat
d) They will travel on the plate the same distance as when a less polar
Choose one of the answers and explain
4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) is
used for stationary phase. An unknown liquid sample is analyzed using paper chromatography using solvent X as the
mobile phase. One spot is observed after the plate is developed and visualized. The same unknown substance is re-
analyzed using solvent Y as the mobile phase. This time, three spots are observed after the plate is developed and
visualized. Is the unknown sample a pure substance or a mixture? Explain your answer, including a possible reason
for the different observations in the two experiments
5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it is
packed into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull.
The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and a
silica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume the
same type of binding forces between the components and the stationary phase are present in both paper
chromatography and column chromatography.)
Transcribed Image Text:1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase than component B. The following result is obtained. Calculate the Rf for each component and label the identity of each spot. 2 Solvent Front 0 0 Spotting Line 2. Which one is the more polar component? Explain 3. If a more polar solvent (mobil phase) is used for the above experiment, the result will be: a) Both component A and Component B will travel further up the plate b) Only Component A will travel further up the plat c) Only Component B will travel further up the plat d) They will travel on the plate the same distance as when a less polar Choose one of the answers and explain 4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) is used for stationary phase. An unknown liquid sample is analyzed using paper chromatography using solvent X as the mobile phase. One spot is observed after the plate is developed and visualized. The same unknown substance is re- analyzed using solvent Y as the mobile phase. This time, three spots are observed after the plate is developed and visualized. Is the unknown sample a pure substance or a mixture? Explain your answer, including a possible reason for the different observations in the two experiments 5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it is packed into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull. The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and a silica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume the same type of binding forces between the components and the stationary phase are present in both paper chromatography and column chromatography.)
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