Sm When proteins are solubilized in inclusion bodies from an E Coli cell lysate, they are unfolded. How do we know from this purification table that the plasminogen has been refolded to its natural (native) state? [Select]
Sm When proteins are solubilized in inclusion bodies from an E Coli cell lysate, they are unfolded. How do we know from this purification table that the plasminogen has been refolded to its natural (native) state? [Select]
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
3
![Answer questions about the following Purification Table
for the purification of plasminogen.
Table 1
Purification of recombinant plasminogen (TAL6003 zymogen) from E coli inclusion bodies.
Purification step
Protein concentration
Total protein
(mg)b
Total activity
(mg)b
Cumulative
Specific
activity
(mg/ml)
activity yield (%)*b
Solubilized inclusion bodies (26 g)
Refolded TAL6003 zymogen
(Post-refold filtrate TAL6003 zymogen)
(Diafiltration retentate TAL6003 zymogen)
(Pre-SP Sepharose filtrate TAL6003 zymogen)
SP Sepharose eluate TAL6003 zymogen
ECH Lysine eluate TAL6003 zymogen
8.88
4,491
5,205
0.51
1,743
1,730
1,542
1,379
0.34 :
0.38
4,354
0.36
3,411
3,313
1,282
1,184
0.49 :
0.33
0.42:
2.31
6.20
1,270
1,195
0.99 :
1.011
When proteins are solubilizeain inclusion bodies from an
E Coli cell lysate, they are unfolded. How do we know
from this purification table that the plasminogen has been
refolded to its natural (native) state?
[ Select ]
Which step of the purification enhances the purity of the
protein the most? [ Select ]
What is the percent yield of plasminogen for the entire
purification? [ Select]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff95edb07-fce6-4fe2-9528-1ac1bc343004%2F688ff7e9-7628-456c-a4d4-d6dc0501c6e2%2F8iwdev_processed.png&w=3840&q=75)
Transcribed Image Text:Answer questions about the following Purification Table
for the purification of plasminogen.
Table 1
Purification of recombinant plasminogen (TAL6003 zymogen) from E coli inclusion bodies.
Purification step
Protein concentration
Total protein
(mg)b
Total activity
(mg)b
Cumulative
Specific
activity
(mg/ml)
activity yield (%)*b
Solubilized inclusion bodies (26 g)
Refolded TAL6003 zymogen
(Post-refold filtrate TAL6003 zymogen)
(Diafiltration retentate TAL6003 zymogen)
(Pre-SP Sepharose filtrate TAL6003 zymogen)
SP Sepharose eluate TAL6003 zymogen
ECH Lysine eluate TAL6003 zymogen
8.88
4,491
5,205
0.51
1,743
1,730
1,542
1,379
0.34 :
0.38
4,354
0.36
3,411
3,313
1,282
1,184
0.49 :
0.33
0.42:
2.31
6.20
1,270
1,195
0.99 :
1.011
When proteins are solubilizeain inclusion bodies from an
E Coli cell lysate, they are unfolded. How do we know
from this purification table that the plasminogen has been
refolded to its natural (native) state?
[ Select ]
Which step of the purification enhances the purity of the
protein the most? [ Select ]
What is the percent yield of plasminogen for the entire
purification? [ Select]
![When proteins are solubilized in inclusion bodies from an
E Coli cell lysate, they are unfolded. How do we know
from this purification table that the plasminogen has been
refolded to its natural (native) state?
[ Select ]
Which step of the purification enhances the purity of the
protein the most? [ Select]
What is the percent yield of asminogen for the entire
purification? [ Select]
What is the fold purification for the entire purification
procedure [Select ]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff95edb07-fce6-4fe2-9528-1ac1bc343004%2F688ff7e9-7628-456c-a4d4-d6dc0501c6e2%2Fse22f_processed.png&w=3840&q=75)
Transcribed Image Text:When proteins are solubilized in inclusion bodies from an
E Coli cell lysate, they are unfolded. How do we know
from this purification table that the plasminogen has been
refolded to its natural (native) state?
[ Select ]
Which step of the purification enhances the purity of the
protein the most? [ Select]
What is the percent yield of asminogen for the entire
purification? [ Select]
What is the fold purification for the entire purification
procedure [Select ]
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