For the following questions (Q1-Q5), consider the compound tartrazine (Yellow 5 food dye) that has a chemical formula of C16H9N4Na3O9S2. Ana weighs out 0.1328 g of tartrazine powder on the balance for her experiment and makes a stock solution by dissolving her powder in 500.0 mL of solution. Concentration (µM or µmol/L)Absorbance0.000.0019.940.25219.90.50629.80.73839.81.018 slope (m): 0.02534 1/(µM)y-intercept (b): -0.0009147 Q5. If Ana determines the absorbance of an unknown tartrazine sample to be 0.662, what is the concentration of the sample in µM?

According to the Beer-Lambert’s Law: where A is the Absorbance, b is the path length of the cuvette…

Answered: slope (m): 0.02534 1/(µM) y-intercept…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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For the following questions (Q1-Q5), consider the compound tartrazine (Yellow 5 food dye) that has a chemical formula of C₁₆H₉N₄Na₃O₉S₂. Ana weighs out 0.1328 g of tartrazine powder on the balance for her experiment and makes a stock solution by dissolving her powder in 500.0 mL of solution.

Concentration (µM or µmol/L)	Absorbance
0.00	0.001
9.94	0.252
19.9	0.506
29.8	0.738
39.8	1.018

slope (m): 0.02534 1/(µM)

y-intercept (b): -0.0009147

Q5. If Ana determines the absorbance of an unknown tartrazine sample to be 0.662, what is the concentration of the sample in µM?

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