Since the Coomassie Brilliant Blue G-250 is a highly (basic, acidic) dye, it cannot be used for proteins with poor (acid, base) solubility.

Principles of Modern Chemistry
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ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter23: Polymeric Materials And Soft Condensed Matter
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Since the Coomassie Brilliant Blue G-250 is a highly (basic, acidic) dye, it cannot be used for proteins with poor (acid, base) solubility.

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