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SigmaS is the stationary phase sigma factor. Which of the following is a likely site for RNAP-Sigmas holoenzyme binding? O The terminator region of genes involved in starvation stress response The -10 and -35 promoter regions of ribosomal RNA genes O The -10 and -35 promoter regions of genes involved in acid stress response The Shine-Delgarno sequence of genes involved in oxidative stress response The CAP operator of the lac operon

SigmaS is the stationary phase sigma factor. Which of the following is a likely site for RNAP-Sigmas holoenzyme binding? O The terminator region of genes involved in starvation stress response The -10 and -35 promoter regions of ribosomal RNA genes O The -10 and -35 promoter regions of genes involved in acid stress response The Shine-Delgarno sequence of genes involved in oxidative stress response The CAP operator of the lac operon

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I need help with both questions, please! 

Sigmas is the stationary phase sigma factor. Which of the following is a likely site for RNAP-Sigmas holoenzyme binding? The terminator region of genes involved in starvation stress response The -10 and -35 promoter regions of ribosomal RNA genes The -10 and -35 promoter regions of genes involved in acid stress response The Shine-Delgarno sequence of genes involved in oxidative stress response The CAP operator of the lac operon
Transcribed Image Text:Sigmas is the stationary phase sigma factor. Which of the following is a likely site for RNAP-Sigmas holoenzyme binding? The terminator region of genes involved in starvation stress response The -10 and -35 promoter regions of ribosomal RNA genes The -10 and -35 promoter regions of genes involved in acid stress response The Shine-Delgarno sequence of genes involved in oxidative stress response The CAP operator of the lac operon
IlvY is a LysR regulator, and it helps control transcription of a neighboring gene, ilvC. IlvY can either bend the DNA and block RNAP's access to ilvC's -35 promoter site, or it can allow access to the ilvC -35 site, thereby promoting ilvC transcription. What determines whether llvY is repressing or activating transcription from the ilvC promoter? The presence of the co-inducer acetolactate - acetolactate binds to IlvY and changes IlvY's shape such that it doesn't block the -35 of the ilvC promoter. IlvY represses ilvC transcription when the IlvC protein is bound to llvY, and it activates ilvC transcription when the llvC protein is not present to bind to llvY. The presence of the co-inducer valine - when valine is present, it binds llvY and causes llvY to repress transcription from the ilvC promoter. The presence of the co-inducer acetolactate - acetolactate induces negative supercoiling at the ilvC promoter, which represses ilvC transcription.
Transcribed Image Text:IlvY is a LysR regulator, and it helps control transcription of a neighboring gene, ilvC. IlvY can either bend the DNA and block RNAP's access to ilvC's -35 promoter site, or it can allow access to the ilvC -35 site, thereby promoting ilvC transcription. What determines whether llvY is repressing or activating transcription from the ilvC promoter? The presence of the co-inducer acetolactate - acetolactate binds to IlvY and changes IlvY's shape such that it doesn't block the -35 of the ilvC promoter. IlvY represses ilvC transcription when the IlvC protein is bound to llvY, and it activates ilvC transcription when the llvC protein is not present to bind to llvY. The presence of the co-inducer valine - when valine is present, it binds llvY and causes llvY to repress transcription from the ilvC promoter. The presence of the co-inducer acetolactate - acetolactate induces negative supercoiling at the ilvC promoter, which represses ilvC transcription.
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Step 1

Sigma factor- 

A sigma factor is a protein needed for initiation of transcription in  bacteria . it is a bacteria transcripition initiation factor that enables specific binding of RNA polymerase to gene promoters. 

the sigma factor ,together with RNA polymerase is known as the RNA polymerase holoenzyme.

the number of sigma factor varies between bacterial species . E.coli has seven sigma factor .

sigma factor are distinguish by their characteristic molecular weight of 70 kDa.

the sigma factor in the RNA polymerase holoenzyme complex is required for the initiation of transcription.it is dissociated from the complex and the RNA continues elongation on its own 

Different sigma factor are utilized under different environment conditions .these specialized sigma factor bind the promoters of genes appropriate to the environmental conditions increasing the transcription of those genes.

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