Sall Kpnl Kpnl 450 600 200 400 1650 1. If you were to subject this digested DNA to agarose gel electrophoresis, what would your gel look like? Draw a detailed picture of your gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. [6]
Sall Kpnl Kpnl 450 600 200 400 1650 1. If you were to subject this digested DNA to agarose gel electrophoresis, what would your gel look like? Draw a detailed picture of your gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. [6]
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![l 74% i 15:53
See the restriction enzyme map below. The total DNA length is 1650 base pairs. If this DNA
is cut using two restriction enzymes, namely Sall and Kpol, it yields four fragments with sizes
of 450 bp. 600 bp, 200 bp and 400 bp. Note that the restriction enzyme cuts this DNA two
times.
Sall
Kpnl Kpnl
450
600
200
400
1650
1. If you were to subject this digested DNA to agarose gel electrophoresis, what would
your gel look like? Draw a detailed picture of your gel. Remember to indicate the
direction in which your DNA is moving and also show any reference samples. Also
remember to show all components of your gel. [6]
Largest
Sall
be
Expected frogment
300
200
S mailes!](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8fe5065b-bd06-4e91-b249-7b212b38ac17%2F7d97789b-ba91-4d4a-8144-448d5ddd5738%2F0qlwbef_processed.jpeg&w=3840&q=75)
Transcribed Image Text:l 74% i 15:53
See the restriction enzyme map below. The total DNA length is 1650 base pairs. If this DNA
is cut using two restriction enzymes, namely Sall and Kpol, it yields four fragments with sizes
of 450 bp. 600 bp, 200 bp and 400 bp. Note that the restriction enzyme cuts this DNA two
times.
Sall
Kpnl Kpnl
450
600
200
400
1650
1. If you were to subject this digested DNA to agarose gel electrophoresis, what would
your gel look like? Draw a detailed picture of your gel. Remember to indicate the
direction in which your DNA is moving and also show any reference samples. Also
remember to show all components of your gel. [6]
Largest
Sall
be
Expected frogment
300
200
S mailes!
![" l 74% I 15:54
H
2. You are provided with coiled DNA and plasmid DNA that you subject to gel
electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA
is moving and also show any reference samples. Also remember to show all
components of your gel. Exact fragment sizes are not important.
[3]
3. How would the bands on your gel appear if the voltage was too high during your
experiment?
[1
a high voltage can heat up the buffer and cause the gel to melt a high voltage can heat up the
buffer and cause the gel to melt
||
טך](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8fe5065b-bd06-4e91-b249-7b212b38ac17%2F7d97789b-ba91-4d4a-8144-448d5ddd5738%2F6kdy5xr_processed.jpeg&w=3840&q=75)
Transcribed Image Text:" l 74% I 15:54
H
2. You are provided with coiled DNA and plasmid DNA that you subject to gel
electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA
is moving and also show any reference samples. Also remember to show all
components of your gel. Exact fragment sizes are not important.
[3]
3. How would the bands on your gel appear if the voltage was too high during your
experiment?
[1
a high voltage can heat up the buffer and cause the gel to melt a high voltage can heat up the
buffer and cause the gel to melt
||
טך
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