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Ruminococcaceae (%) PC2: 9.32% Variance 0.2 0.0 C -0.40.4 1.00- Actinobacteria (%) 0.75- 0.50 0.25 -0.2 0.0 Condition Hothy Cromar 1000- 0.2 0.4 PC1: 13.8 % Variance 0.00 Healthy CF 60- 20- Healthy CF Sutterella (%) Butyricimonas (%) 20 15. 10- Alpha Diversity (Chao1) 800- 600 400 Healthy CF Таха Acidaminococcus Clostridium Tissierellaceae- Vellonella- Clostridiaceae- Fusobacteriaceae- Fusobacteria- Fusobacteriales- Streptococcaceae- Lactobacillales- Bacill Firmicutes Actinobacteria- Ruminococcacene Caprococcus Christensenellaceae- Barnesiellaceae- Alcaligenaceae- Verrucomicrobia Down in CF Up in CF Verrucomicrobiales- Verrucomicrobiaceae- Verrucomicrobiae- Sutterella- Cyanobacteria- Deltaproteobacteria- Butyricimonas- Bilophila Phylum Bacter Promobacteria Funclectora Actinctontwr Desulfovibrionaceae- Desulfovibrionales- D Healthy CF 1.00- Healthy CF Actinobacteria (%) 0.75 0.50- 0.25 -3 0 في Cynobacteris log2(Fold Change) 0.00- Healthy CF(Other) CFTR Genotype CF(df508) Fig. 2 Differences between cystic fibrosis (CF) and healthy gut mucosal microbiota. a (left) Principal coordinate analysis plot based on Bray-Curtis distance indicating difference in beta-diversity between CF and healthy gut mucosal microbiome. The axes represent the percentage variance along the first two principal components and the color of samples indicates their mutation status, ie., Healthy, CF (other), and CF (df508); (right) Boxplot depicting difference in alpha diversity (Chaol metric) between CF and healthy gut microbiome. b Dotplot showing significantly differentially abundant OTUS (q value <0.1), where OTUS are grouped by genera along the y-axis and colored by phylum. The x-axis indicates the log2 fold-change in CF compared to healthy as baseline. c Boxplots indicating the percentage relative abundance of taxa showing differential abundance between CF and healthy gut microbiome (q value <0.1). d Boxplot depicting gradient-like trend in abundance for Actinobacteria for three genotypes Healthy, CF (other), and CF (df508) APR 4 M tv ST A W MacBook Pro

Ruminococcaceae (%) PC2: 9.32% Variance 0.2 0.0 C -0.40.4 1.00- Actinobacteria (%) 0.75- 0.50 0.25 -0.2 0.0 Condition Hothy Cromar 1000- 0.2 0.4 PC1: 13.8 % Variance 0.00 Healthy CF 60- 20- Healthy CF Sutterella (%) Butyricimonas (%) 20 15. 10- Alpha Diversity (Chao1) 800- 600 400 Healthy CF Таха Acidaminococcus Clostridium Tissierellaceae- Vellonella- Clostridiaceae- Fusobacteriaceae- Fusobacteria- Fusobacteriales- Streptococcaceae- Lactobacillales- Bacill Firmicutes Actinobacteria- Ruminococcacene Caprococcus Christensenellaceae- Barnesiellaceae- Alcaligenaceae- Verrucomicrobia Down in CF Up in CF Verrucomicrobiales- Verrucomicrobiaceae- Verrucomicrobiae- Sutterella- Cyanobacteria- Deltaproteobacteria- Butyricimonas- Bilophila Phylum Bacter Promobacteria Funclectora Actinctontwr Desulfovibrionaceae- Desulfovibrionales- D Healthy CF 1.00- Healthy CF Actinobacteria (%) 0.75 0.50- 0.25 -3 0 في Cynobacteris log2(Fold Change) 0.00- Healthy CF(Other) CFTR Genotype CF(df508) Fig. 2 Differences between cystic fibrosis (CF) and healthy gut mucosal microbiota. a (left) Principal coordinate analysis plot based on Bray-Curtis distance indicating difference in beta-diversity between CF and healthy gut mucosal microbiome. The axes represent the percentage variance along the first two principal components and the color of samples indicates their mutation status, ie., Healthy, CF (other), and CF (df508); (right) Boxplot depicting difference in alpha diversity (Chaol metric) between CF and healthy gut microbiome. b Dotplot showing significantly differentially abundant OTUS (q value <0.1), where OTUS are grouped by genera along the y-axis and colored by phylum. The x-axis indicates the log2 fold-change in CF compared to healthy as baseline. c Boxplots indicating the percentage relative abundance of taxa showing differential abundance between CF and healthy gut microbiome (q value <0.1). d Boxplot depicting gradient-like trend in abundance for Actinobacteria for three genotypes Healthy, CF (other), and CF (df508) APR 4 M tv ST A W MacBook Pro

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Which phylum of bacteria are most frequently unregulated and downregulated in CF samples?
Ruminococcaceae (%) PC2: 9.32% Variance 0.2 0.0 C -0.40.4 1.00- Actinobacteria (%) 0.75- 0.50 0.25 -0.2 0.0 Condition Hothy Cromar 1000- 0.2 0.4 PC1: 13.8 % Variance 0.00 Healthy CF 60- 20- Healthy CF Sutterella (%) Butyricimonas (%) 20 15. 10- Alpha Diversity (Chao1) 800- 600 400 Healthy CF Таха Acidaminococcus Clostridium Tissierellaceae- Vellonella- Clostridiaceae- Fusobacteriaceae- Fusobacteria- Fusobacteriales- Streptococcaceae- Lactobacillales- Bacill Firmicutes Actinobacteria- Ruminococcacene Caprococcus Christensenellaceae- Barnesiellaceae- Alcaligenaceae- Verrucomicrobia Down in CF Up in CF Verrucomicrobiales- Verrucomicrobiaceae- Verrucomicrobiae- Sutterella- Cyanobacteria- Deltaproteobacteria- Butyricimonas- Bilophila Phylum Bacter Promobacteria Funclectora Actinctontwr Desulfovibrionaceae- Desulfovibrionales- D Healthy CF 1.00- Healthy CF Actinobacteria (%) 0.75 0.50- 0.25 -3 0 في Cynobacteris log2(Fold Change) 0.00- Healthy CF(Other) CFTR Genotype CF(df508) Fig. 2 Differences between cystic fibrosis (CF) and healthy gut mucosal microbiota. a (left) Principal coordinate analysis plot based on Bray-Curtis distance indicating difference in beta-diversity between CF and healthy gut mucosal microbiome. The axes represent the percentage variance along the first two principal components and the color of samples indicates their mutation status, ie., Healthy, CF (other), and CF (df508); (right) Boxplot depicting difference in alpha diversity (Chaol metric) between CF and healthy gut microbiome. b Dotplot showing significantly differentially abundant OTUS (q value <0.1), where OTUS are grouped by genera along the y-axis and colored by phylum. The x-axis indicates the log2 fold-change in CF compared to healthy as baseline. c Boxplots indicating the percentage relative abundance of taxa showing differential abundance between CF and healthy gut microbiome (q value <0.1). d Boxplot depicting gradient-like trend in abundance for Actinobacteria for three genotypes Healthy, CF (other), and CF (df508) APR 4 M tv ST A W MacBook Pro
Transcribed Image Text:Ruminococcaceae (%) PC2: 9.32% Variance 0.2 0.0 C -0.40.4 1.00- Actinobacteria (%) 0.75- 0.50 0.25 -0.2 0.0 Condition Hothy Cromar 1000- 0.2 0.4 PC1: 13.8 % Variance 0.00 Healthy CF 60- 20- Healthy CF Sutterella (%) Butyricimonas (%) 20 15. 10- Alpha Diversity (Chao1) 800- 600 400 Healthy CF Таха Acidaminococcus Clostridium Tissierellaceae- Vellonella- Clostridiaceae- Fusobacteriaceae- Fusobacteria- Fusobacteriales- Streptococcaceae- Lactobacillales- Bacill Firmicutes Actinobacteria- Ruminococcacene Caprococcus Christensenellaceae- Barnesiellaceae- Alcaligenaceae- Verrucomicrobia Down in CF Up in CF Verrucomicrobiales- Verrucomicrobiaceae- Verrucomicrobiae- Sutterella- Cyanobacteria- Deltaproteobacteria- Butyricimonas- Bilophila Phylum Bacter Promobacteria Funclectora Actinctontwr Desulfovibrionaceae- Desulfovibrionales- D Healthy CF 1.00- Healthy CF Actinobacteria (%) 0.75 0.50- 0.25 -3 0 في Cynobacteris log2(Fold Change) 0.00- Healthy CF(Other) CFTR Genotype CF(df508) Fig. 2 Differences between cystic fibrosis (CF) and healthy gut mucosal microbiota. a (left) Principal coordinate analysis plot based on Bray-Curtis distance indicating difference in beta-diversity between CF and healthy gut mucosal microbiome. The axes represent the percentage variance along the first two principal components and the color of samples indicates their mutation status, ie., Healthy, CF (other), and CF (df508); (right) Boxplot depicting difference in alpha diversity (Chaol metric) between CF and healthy gut microbiome. b Dotplot showing significantly differentially abundant OTUS (q value <0.1), where OTUS are grouped by genera along the y-axis and colored by phylum. The x-axis indicates the log2 fold-change in CF compared to healthy as baseline. c Boxplots indicating the percentage relative abundance of taxa showing differential abundance between CF and healthy gut microbiome (q value <0.1). d Boxplot depicting gradient-like trend in abundance for Actinobacteria for three genotypes Healthy, CF (other), and CF (df508) APR 4 M tv ST A W MacBook Pro
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