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RNase is an enzyme that cleaves the P-05 bond in RNA. It has two His residues in the active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from pH 6.0, as shown in the graph. Activity (%) 120 100 80 60 40 20 0 0 10 PH Data provided by Dr. Sunita Chowrira, University of British Columbia. Choose all of the true statements. There are two His residues and both are deprotonated below pH 4.2. The substrate is affected by the change in pH and is inactive (postively charged) when pH is above 6.0. RNAse is an acid-base catalyst. The RNase reaction is an example of metal ion catalysis with a positively charged metal. Both His residues are deprotonated at pH higher than than 6.5.

RNase is an enzyme that cleaves the P-05 bond in RNA. It has two His residues in the active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from pH 6.0, as shown in the graph. Activity (%) 120 100 80 60 40 20 0 0 10 PH Data provided by Dr. Sunita Chowrira, University of British Columbia. Choose all of the true statements. There are two His residues and both are deprotonated below pH 4.2. The substrate is affected by the change in pH and is inactive (postively charged) when pH is above 6.0. RNAse is an acid-base catalyst. The RNase reaction is an example of metal ion catalysis with a positively charged metal. Both His residues are deprotonated at pH higher than than 6.5.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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RNase is an enzyme that cleaves the P-05 bond in RNA. It has two His residues in the active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from pH 6.0, as shown in the graph. Activity (%) 120 100 80 60 40 20 0 0 10 PH Data provided by Dr. Sunita Chowrira, University of British Columbia. Choose all of the true statements. There are two His residues and both are deprotonated below pH 4.2. The substrate is affected by the change in pH and is inactive (postively charged) when pH is above 6.0. RNAse is an acid-base catalyst. The RNase reaction is an example of metal ion catalysis with a positively charged metal. Both His residues are deprotonated at pH higher than than 6.5.
Transcribed Image Text:RNase is an enzyme that cleaves the P-05 bond in RNA. It has two His residues in the active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from pH 6.0, as shown in the graph. Activity (%) 120 100 80 60 40 20 0 0 10 PH Data provided by Dr. Sunita Chowrira, University of British Columbia. Choose all of the true statements. There are two His residues and both are deprotonated below pH 4.2. The substrate is affected by the change in pH and is inactive (postively charged) when pH is above 6.0. RNAse is an acid-base catalyst. The RNase reaction is an example of metal ion catalysis with a positively charged metal. Both His residues are deprotonated at pH higher than than 6.5.
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