In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Procedure:A. Peptic digestion1. Prepare 4 test tubes as follows:Tube 1 - 3 ml pepsinTube 2 - 3 ml of 0.4% HC1Tube 3 - 3 ml pepsin +| 1.5 ml of 0.4% HClTube 4 - 3 ml pepsin + 1.5 ml of 1% NazCO32. Add equal slices of hard-boiled egg white to each tube.3. Cover and incubate at 40°C for 2 hours.4. Add 4 drops of toluene and store till the next lab period.5. Determine the extent of digestion by visually noting the size of the hard-boiled eggwhite in each tube.6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate testtubes labelled tube la. 2a, 3a, 4a.7. Neutralize tubes 2a& 3a with solid sodium carbonate.8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO. Comparethe colors obtained in the 4 tubes.9. Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2 drops of1% CuSO4. Compare the result of these tubes with the other 4 tubes above.B. Pancreatin digestion1. Prepare 4 test tubes as follows:Tube 1 - 3 ml neutral pancreatinTube 2 - 3 ml waterTube 3 - 3 ml water +1.5 ml pancreatinTube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% NaCO;2. Place equal size of hard-boiled egg white in all tubes.3. Place all tubes in water bath with a temperature maintained at 40°C for 1.5 hours.4. Add 3 drops of toluene in all tubes and incubate till the next lab period.5. Observe the size of the egg white in each of the tubes.6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each tube. Mix andcompare the result of the 4 tubes. RESULTS:A. Peptic DigestionTUBESIZE of egg whiteResultsno change in sizeviolet solution1 (реpsin)light violet solutionblue solutionviolet solution2 (HCI)minimal changebecame smallerno change in size3(pepsin, HCI)4(pepsin, Na:CO:)5 PEPΤONEdark violet solutionB. Pancreatin DigestionTUBESIZE of egg whiteno change in sizeno change in sizeno change in sizeResults1(Pancreatin)2(Water)3(water, Pancreatin)4(water, Pancreatin,Na,CO;)violet solutionviolet solutionviolet solutionblue solutionbecame smaller1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatindigestion?PepticPancreatin

Proteins are polypeptides composed of individual amino acids. Amino acids are molecules that possess…

RESULTS: A. Peptic Digestion TUBE SIZE of egg white Results 1 (pepsin) no change in size violet solution minimal change became smaller no change in size light violet solution blue solution violet solution dark violet solution 2 (HCI) 3(pepsin, HCI) 4(pepsin, NazCO:) 5 ΡΕPTONΕ B. Pancreatin Digestion TUBE 1(Pancreatin) 2(Water) 3(water, Pancreatin) 4(water, Pancreatin, | Na:CO;) SIZE of egg white no change in size no change in size no change in size became smaller Results violet solution violet solution violet solution blue solution 1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Peptic Pancreatin

RESULTS: A. Peptic Digestion TUBE SIZE of egg white Results 1 (pepsin) no change in size violet solution minimal change became smaller no change in size light violet solution blue solution violet solution dark violet solution 2 (HCI) 3(pepsin, HCI) 4(pepsin, NazCO:) 5 ΡΕPTONΕ B. Pancreatin Digestion TUBE 1(Pancreatin) 2(Water) 3(water, Pancreatin) 4(water, Pancreatin, | Na:CO;) SIZE of egg white no change in size no change in size no change in size became smaller Results violet solution violet solution violet solution blue solution 1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Peptic Pancreatin

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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