Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
RESULTS: A. Peptic Digestion TUBE SIZE of egg white Results 1 (pepsin) no change in size violet solution minimal change became smaller no change in size light violet solution blue solution violet solution dark violet solution 2 (HCI) 3(pepsin, HCI) 4(pepsin, NazCO:) 5 ΡΕPTONΕ B. Pancreatin Digestion TUBE 1(Pancreatin) 2(Water) 3(water, Pancreatin) 4(water, Pancreatin, | Na:CO;) SIZE of egg white no change in size no change in size no change in size became smaller Results violet solution violet solution violet solution blue solution 1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Peptic Pancreatin

RESULTS: A. Peptic Digestion TUBE SIZE of egg white Results 1 (pepsin) no change in size violet solution minimal change became smaller no change in size light violet solution blue solution violet solution dark violet solution 2 (HCI) 3(pepsin, HCI) 4(pepsin, NazCO:) 5 ΡΕPTONΕ B. Pancreatin Digestion TUBE 1(Pancreatin) 2(Water) 3(water, Pancreatin) 4(water, Pancreatin, | Na:CO;) SIZE of egg white no change in size no change in size no change in size became smaller Results violet solution violet solution violet solution blue solution 1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Peptic Pancreatin

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
  1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion?

                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

Procedure: A. Peptic digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml pepsin Tube 2 - 3 ml of 0.4% HC1 Tube 3 - 3 ml pepsin +| 1.5 ml of 0.4% HCl Tube 4 - 3 ml pepsin + 1.5 ml of 1% NazCO3 2. Add equal slices of hard-boiled egg white to each tube. 3. Cover and incubate at 40°C for 2 hours. 4. Add 4 drops of toluene and store till the next lab period. 5. Determine the extent of digestion by visually noting the size of the hard-boiled egg white in each tube. 6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate test tubes labelled tube la. 2a, 3a, 4a. 7. Neutralize tubes 2a& 3a with solid sodium carbonate. 8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO. Compare the colors obtained in the 4 tubes. 9. Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2 drops of 1% CuSO4. Compare the result of these tubes with the other 4 tubes above. B. Pancreatin digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml neutral pancreatin Tube 2 - 3 ml water Tube 3 - 3 ml water +1.5 ml pancreatin Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% NaCO; 2. Place equal size of hard-boiled egg white in all tubes. 3. Place all tubes in water bath with a temperature maintained at 40°C for 1.5 hours. 4. Add 3 drops of toluene in all tubes and incubate till the next lab period. 5. Observe the size of the egg white in each of the tubes. 6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each tube. Mix and compare the result of the 4 tubes.
Transcribed Image Text:Procedure: A. Peptic digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml pepsin Tube 2 - 3 ml of 0.4% HC1 Tube 3 - 3 ml pepsin +| 1.5 ml of 0.4% HCl Tube 4 - 3 ml pepsin + 1.5 ml of 1% NazCO3 2. Add equal slices of hard-boiled egg white to each tube. 3. Cover and incubate at 40°C for 2 hours. 4. Add 4 drops of toluene and store till the next lab period. 5. Determine the extent of digestion by visually noting the size of the hard-boiled egg white in each tube. 6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate test tubes labelled tube la. 2a, 3a, 4a. 7. Neutralize tubes 2a& 3a with solid sodium carbonate. 8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO. Compare the colors obtained in the 4 tubes. 9. Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2 drops of 1% CuSO4. Compare the result of these tubes with the other 4 tubes above. B. Pancreatin digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml neutral pancreatin Tube 2 - 3 ml water Tube 3 - 3 ml water +1.5 ml pancreatin Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% NaCO; 2. Place equal size of hard-boiled egg white in all tubes. 3. Place all tubes in water bath with a temperature maintained at 40°C for 1.5 hours. 4. Add 3 drops of toluene in all tubes and incubate till the next lab period. 5. Observe the size of the egg white in each of the tubes. 6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each tube. Mix and compare the result of the 4 tubes.
RESULTS: A. Peptic Digestion TUBE SIZE of egg white Results no change in size violet solution 1 (реpsin) light violet solution blue solution violet solution 2 (HCI) minimal change became smaller no change in size 3(pepsin, HCI) 4(pepsin, Na:CO:) 5 PEPΤONE dark violet solution B. Pancreatin Digestion TUBE SIZE of egg white no change in size no change in size no change in size Results 1(Pancreatin) 2(Water) 3(water, Pancreatin) 4(water, Pancreatin, Na,CO;) violet solution violet solution violet solution blue solution became smaller 1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Peptic Pancreatin
Transcribed Image Text:RESULTS: A. Peptic Digestion TUBE SIZE of egg white Results no change in size violet solution 1 (реpsin) light violet solution blue solution violet solution 2 (HCI) minimal change became smaller no change in size 3(pepsin, HCI) 4(pepsin, Na:CO:) 5 PEPΤONE dark violet solution B. Pancreatin Digestion TUBE SIZE of egg white no change in size no change in size no change in size Results 1(Pancreatin) 2(Water) 3(water, Pancreatin) 4(water, Pancreatin, Na,CO;) violet solution violet solution violet solution blue solution became smaller 1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion? Peptic Pancreatin
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS