Questions 5. Use Figure 5 above to complete the following: a. Using a highlighter (any color), highlight the following components: flavoprotein, quinone, and cytochrome. b. Circle the protons. c. Draw boxes around the electrons. 6. In Figure 5 does a proton or an electron cross the membrane first? 7. Is the cytochrome translocating protons or electrons? 8. In Figure 5, how many ATPs are made via the spatially positioned system? 9. Do you know how many ATPs scientists currently think are made with the electron transport chain?

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Chapter1: Biochemistry: An Evolving Science
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Mitchell knew that different metabolic inhibitors could inhibit oxidative phosphorylation, and he tentatively
identified some of the components in his hypothetical system (Figure 5). He hypothesized that a
flavoprotein, a quinone, and a cytochrome were important in oxidative phosphorylation. He placed these
macromolecules within or adjacent to his membrane, based on the knowledge at the time. Please note
that the notation for some molecules was different in 1961.
Inside (L)
Membrane
SH₂
S+H*
DPN
NAD
OPNH-
QH₂
9+2H*
30H'<
e+H>
20
2.H
20
Cyt.-
Outside (R)
FP+H*
FAD
FPH₂
0+ H*
OH'
3(ADP+P)
H*
3ATP
Figure 5. The original caption for this figure in Mitchell (1961) reads: "Eig. 3. Diagram of chemi-osmotic sys-
tem for coupling phosphorylation to the oxidation of substrate (SH) through DPN, FP (tentatively identified
with flavoprotein), Q (tentatively identified with a quinone) and the cytochromes (Cyr.)." Credit: Reprinted by
permission from Macmillan Publishers Ltd: Nature ©1961.
Transcribed Image Text:Mitchell knew that different metabolic inhibitors could inhibit oxidative phosphorylation, and he tentatively identified some of the components in his hypothetical system (Figure 5). He hypothesized that a flavoprotein, a quinone, and a cytochrome were important in oxidative phosphorylation. He placed these macromolecules within or adjacent to his membrane, based on the knowledge at the time. Please note that the notation for some molecules was different in 1961. Inside (L) Membrane SH₂ S+H* DPN NAD OPNH- QH₂ 9+2H* 30H'< e+H> 20 2.H 20 Cyt.- Outside (R) FP+H* FAD FPH₂ 0+ H* OH' 3(ADP+P) H* 3ATP Figure 5. The original caption for this figure in Mitchell (1961) reads: "Eig. 3. Diagram of chemi-osmotic sys- tem for coupling phosphorylation to the oxidation of substrate (SH) through DPN, FP (tentatively identified with flavoprotein), Q (tentatively identified with a quinone) and the cytochromes (Cyr.)." Credit: Reprinted by permission from Macmillan Publishers Ltd: Nature ©1961.
Questions
5. Use Figure 5 above to complete the following:
a. Using a highlighter (any color), highlight the following components: flavoprotein, quinone,
and cytochrome.
b. Circle the protons.
c. Draw boxes around the electrons.
6. In Figure 5 does a proton or an electron cross the membrane first?
7. Is the cytochrome translocating protons or electrons?
8. In Figure 5, how many ATPs are made via the spatially positioned system?
9. Do you know how many ATPs scientists currently think are made with the electron transport chain?
Transcribed Image Text:Questions 5. Use Figure 5 above to complete the following: a. Using a highlighter (any color), highlight the following components: flavoprotein, quinone, and cytochrome. b. Circle the protons. c. Draw boxes around the electrons. 6. In Figure 5 does a proton or an electron cross the membrane first? 7. Is the cytochrome translocating protons or electrons? 8. In Figure 5, how many ATPs are made via the spatially positioned system? 9. Do you know how many ATPs scientists currently think are made with the electron transport chain?
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