Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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*Short Response*

Transcribed Image Text:Question 9. Which protein separation technique
depends on the ability of charged molecules to
migrate if placed in an electric field?
Expert Solution

Step 1: Introduction
Electrophoresis is defined as the migration of charged particles under the influence of electric field. An electrophoretic system consists of two electrodes of opposite charge (anode, cathode), connected by a conducting medium called an electrolyte. Factors affecting electrophoresis are-
1. Size of ion/ molecule
2. Charge of ion/ molecule
3. Temperature
4. Ionic strength of the buffer
5. Potential difference/voltage applied
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