Question 9. Which protein separation technique depends on the ability of charged molecules to migrate if placed in an electric field?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Question 9. Which protein separation technique
depends on the ability of charged molecules to
migrate if placed in an electric field?
Transcribed Image Text:Question 9. Which protein separation technique depends on the ability of charged molecules to migrate if placed in an electric field?
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Step 1: Introduction

Electrophoresis is defined as the migration of charged particles under the influence of electric field.  An electrophoretic system consists of two electrodes of opposite charge (anode, cathode), connected by a conducting medium called an electrolyte. Factors affecting electrophoresis are-

1. Size of ion/ molecule

2. Charge of ion/ molecule

3. Temperature

4. Ionic strength of the buffer

5. Potential difference/voltage applied

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