Question 5 You are a student researcher in a comparative physiology lab for the summer. You are interested in temperature effects on lactate dehydrogenase (LDH) in fish. Your supervisor has asked you to write a hypothesis and prediction before you start your experiments. Choose the answer below that is most likely, based on the scientific literature (and what you have learned in BIOL*1070). Hypothesis: Temperature changes impact enzyme function through homeoviscous adaptation. Prediction: If so, then LDH activity will be maintained at all temperatures. Hypothesis: Ectotherms adjust enzyme expression during chronic temperature change to maintain enzyme function. Prediction: If so, then LDH activity in fish acclimatized to warmer temperatures will be approximately the same as fish acclimatized to cooler temperatures. O Hypothesis: Ectotherms conform to their environment. Prediction: If so, then LDH activity in fish will increase in warm waters and decrease in cool waters at all time points. O Hypothesis: Warm temperatures accelerate enzyme rates. Prediction: If so, then LDH activity in fish acclimatized to warmer temperatures will always be higher relative to fish acclimatized to cooler temperatures.

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