Question #19: The enzyme-linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying the presence of an antigen in a sample. This assay involves several steps. What would happen if you skipped the step of blocking unoccupied sites with nonspecific protein before adding the primary antibody? A) None of the other answers is correct B) The accuracy of the assay would increase because the primary antibody wouldn't bind to the nonspecific protein anymore c) The result would be the same because the primary antibody would still bind O only to the antigen D)The result would be inaccurate because the primary antibody would stick to the upoccupied sites, ultimately leading to excessive product formation E) There would be no colorimetric product because the reporter enzyme would specifically stick to the unoccupied sites and become denatured. Question # 20: Which of the following is FALSE about glycosaminoglycans (GAGS)? A) They contribute to the structure of the extracellular matrix between cells B) BThey are enriched in negative charges C) C) They can be attached to extracellular domains of proteins via a short peptide linker to form proteoglycans tails D))They are attached to every available Ser residue in extracellular regions of proteins E) A GAG called heparin is useful as a mimic of DNA * Vmex E)
Question #19: The enzyme-linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying the presence of an antigen in a sample. This assay involves several steps. What would happen if you skipped the step of blocking unoccupied sites with nonspecific protein before adding the primary antibody? A) None of the other answers is correct B) The accuracy of the assay would increase because the primary antibody wouldn't bind to the nonspecific protein anymore c) The result would be the same because the primary antibody would still bind O only to the antigen D)The result would be inaccurate because the primary antibody would stick to the upoccupied sites, ultimately leading to excessive product formation E) There would be no colorimetric product because the reporter enzyme would specifically stick to the unoccupied sites and become denatured. Question # 20: Which of the following is FALSE about glycosaminoglycans (GAGS)? A) They contribute to the structure of the extracellular matrix between cells B) BThey are enriched in negative charges C) C) They can be attached to extracellular domains of proteins via a short peptide linker to form proteoglycans tails D))They are attached to every available Ser residue in extracellular regions of proteins E) A GAG called heparin is useful as a mimic of DNA * Vmex E)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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I wanna check for my answer correct or no (19 & 20) please
Thank you
Transcribed Image Text:Question #19: The enzyme-linked immunosorbent assay (ELISA) is a powerful methỏd
for detecting and quantifying the presence of an antigen in a sample. This assay involves
several steps. What would happen if you skipped the step of blocking unoccupied sites
with nonspecific protein before adding the primary antibody?
A) None of the other answers is correct
B) The accuracy of the assay would increase because the primary antibody wouldn't bind to the
nonspecific protein anymore
c) The result would be the same because the primary antibody would still bind O only to the
antigen
D))The result would be inaccurate because the primary antibody would stick to the upoccupied
sites, ultimately leading to excessive product formation
E) There would be no colorimetric product because the reporter enzyme would specifically stick
to the unoccupied sites and become denatured.
Question # 20: Which of the following is FALSE about glycosaminoglycans (GAGS)?
A) They contribute to the structure of the extracellular matrix between cells
B) BThey are enriched in negative charges
C) C) They can be attached to extracellular domains of proteins via a short peptide linker to
form proteoglycans
D)) They are attached to every available Ser residue in extracellular regions of proteins
E) . E) A GAG called heparin is useful as a mimic of DNA * Vmex
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