18 please QUESTION 17A student wanted to run a protein sample on a SDS PAGE gel, and in the process of casting a gel the student had forgotten to add the stacking layer on top of theresolving layer of the gel. What problems would likely arise as a result of this mishap?Proteins would migrate much faster through the gelProteins would not be able to migrate through the resolving layer and stays at the top of the gelProteins will move into the resolving layer at different times and this would result in a smear on the gelThere is no problem, proteins in the sample would resolve the same as if they were ran on a SDS PAGE gel with a stacking layer on top of a resolving layerQUESTION 18Sample A is diluted by adding 50 ul to 950 uL of buffer to make solution B. Solution B is diluted by adding 50 uL of solution B to 300 uL of buffer.The absorbance of sample C is 0.05. What is the concentration of sample A assuming the following parameters.|=1 cmMolar extinction coefficient = 10 M-1 cm-1O 0.7 MO 0.8 MO 0.9 MO 1.0 M

The relation between the concentration of solution and absorbance is given by Beer Lambert's law.…

QUESTION 18 Sample A is diluted by adding 50 uL to 950 ul of buffer to make solution B. Solution B is diluted by adding 50 uL of solution B to 300 uL of buffer. The absorbance of sample C is 0.05. What is the concentration of sample A assuming the following parameters. |= 1 cm Molar extinction coefficient = 10 M-1 cm-1 O 0.7 M 0.8 M O 0.9 M O 1.0 M

QUESTION 18 Sample A is diluted by adding 50 uL to 950 ul of buffer to make solution B. Solution B is diluted by adding 50 uL of solution B to 300 uL of buffer. The absorbance of sample C is 0.05. What is the concentration of sample A assuming the following parameters. |= 1 cm Molar extinction coefficient = 10 M-1 cm-1 O 0.7 M 0.8 M O 0.9 M O 1.0 M

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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